THE EFFECT OF SOME CHEMICAL FACTORS ON SERINOPROTEINASES ACTIVITY OF TRYPSIN AND CHYMOTRYPSIN IN CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS) FRY

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ZAMANI ABBAS

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Journal: JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST) Year:2016 | Volume:4 | Issue:4 Page(s): 51-63

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Title

THE EFFECT OF SOME CHEMICAL FACTORS ON SERINOPROTEINASES ACTIVITY OF TRYPSIN AND CHYMOTRYPSIN IN CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS) FRY

Author(s)

ZAMANI ABBAS | Issue Writer Certificate

Keywords

FRYQ2

CASPIAN BROWN TROUTQ2

CHEMICAL FACTORSQ2

CHYMOTRYPSINQ2

Abstract

The effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors was considered on trypsin and CHYMOTRYPSIN activity of the CASPIAN BROWN TROUT FRY. The results showed K+ and Na+ didn’t significanly decrease trypsin and CHYMOTRYPSIN activity (p>0.05). Ca2+ and Mg2+ significantly increased trypsin and CHYMOTRYPSIN activity (p<0.05). Mn2+,Cu2+,Ba2+,Co2+,Zn2+,Fe2+ and Al3+ significantly decreased the activity of trypsin and CHYMOTRYPSIN (p<0.05). Saponin and taurocholic acid significantly increased trypsin and CHYMOTRYPSIN activity. Sodium cholate significantly increased CHYMOTRYPSIN activity (p<0.05), but not the trypsin activity (p>0.05). Oxidizing agents, including hydrogen peroxide and sodium perborate, significantly decreased trypsin (p<0.05). Trypsin and CHYMOTRYPSIN activity significantly decreased in the presence of SBTI, PMSF and r-Aminobenzamidine inhibitors (p>0.05). The inhibitors such as TPCK, pepstatinA, iodoacetic acid, EDTA and b-mercaptoethanol did not significantly decrease the trypsin activity (p>0.05), but they significantly decreased CHYMOTRYPSIN activity (p<0.05). Trypsin activity in the presence of TLCK showed a significant decrease (p<0.05), but TLCK and pepstatin A had no significant effect on CHYMOTRYPSIN activity (p>0.05).

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APA: Copy

ZAMANI, ABBAS. (2016). THE EFFECT OF SOME CHEMICAL FACTORS ON SERINOPROTEINASES ACTIVITY OF TRYPSIN AND CHYMOTRYPSIN IN CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS) FRY. JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST), 4(4), 51-63. SID. https://sid.ir/paper/246799/en

Vancouver: Copy

ZAMANI ABBAS. THE EFFECT OF SOME CHEMICAL FACTORS ON SERINOPROTEINASES ACTIVITY OF TRYPSIN AND CHYMOTRYPSIN IN CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS) FRY. JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST)[Internet]. 2016;4(4):51-63. Available from: https://sid.ir/paper/246799/en

IEEE: Copy

ABBAS ZAMANI, “THE EFFECT OF SOME CHEMICAL FACTORS ON SERINOPROTEINASES ACTIVITY OF TRYPSIN AND CHYMOTRYPSIN IN CASPIAN BROWN TROUT (SALMO TRUTTA CASPIUS) FRY,” JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST), vol. 4, no. 4, pp. 51–63, 2016, [Online]. Available: https://sid.ir/paper/246799/en

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