IDENTIFICATION OF SELF- INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY DEGENERATE S-RNASE PRIMERS

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KAMAREH A.; SHARAFI Y.; GHANBARI A.

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Journal: Journal of Nuts Year:2015 | Volume:6 | Issue:2 Page(s): 165-172

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Information Journal Paper

Title

IDENTIFICATION OF SELF- INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY DEGENERATE S-RNASE PRIMERS

Author(s)

KAMAREH A. | SHARAFI Y. | GHANBARI A. | Issue Writer Certificate

Keywords

ALMONDQ2

INCOMPATIBILITYQ2

PCRQ2

S-ALLELESQ2

Abstract

The ALMOND, Prunus dulcis Miller which belongs to Rosaceae family, is one of the most important commercial and oldest cultivated tree nut crops. ALMONDs are classified as a ‘nut’ in which the edible seed is the commercial product. Therefore, pollination and fertilization are necessary in ALMOND. The characteristic of cultivated ALMOND to express gametophytic self- INCOMPATIBILITY discourages self-fertilization and favors cross pollination. Genetic control of pollen- pistil self-incompatibility is through a single gene (S) which exists in a series of alleles S1 to Sx. Compatibility of pollen-pistil in ALMOND is an important consideration in planning crosses in breeding program and in choosing pollinizers for orchard planting. Identification of self-(in) compatibility in ALMOND carried out by molecular and controlled pollination methods. In this study, identification of S-ALLELES in 37 Iranian ALMOND cultivars and genotypes was carried out by PCR method with using degenerate primers of EM-PC3 cons RDEMPC2 cons FD, PaconsI-Fand EMPC1 cons RD. In this way the size of S -alleles were estimated based on bands which amplified with second intron. The results confirmed self-incompatibility in cultivars and most genotypes. However, the Sf-like allele (in size) was observed in A9 and A36 genotypes. If these results are confirmed by sequencing the Sf allele, it will be first time to identify self-compatible genotype in Iranian ALMOND genotypes.

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APA: Copy

KAMAREH, A., SHARAFI, Y., & GHANBARI, A.. (2015). IDENTIFICATION OF SELF- INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY DEGENERATE S-RNASE PRIMERS. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), 6(2 ), 165-172. SID. https://sid.ir/paper/247749/en

Vancouver: Copy

KAMAREH A., SHARAFI Y., GHANBARI A.. IDENTIFICATION OF SELF- INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY DEGENERATE S-RNASE PRIMERS. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES)[Internet]. 2015;6(2 ):165-172. Available from: https://sid.ir/paper/247749/en

IEEE: Copy

A. KAMAREH, Y. SHARAFI, and A. GHANBARI, “IDENTIFICATION OF SELF- INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY DEGENERATE S-RNASE PRIMERS,” JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), vol. 6, no. 2 , pp. 165–172, 2015, [Online]. Available: https://sid.ir/paper/247749/en

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