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Information Journal Paper

Title

Detection of Vibrio parahaemolyticus in shrimp samples collected from Ardabil by PCR method

Pages

  26-35

Abstract

 Vibrio parahaemolyticus, a member of family Vibrionaceae, is a non-spore-forming, rodshaped, gram-negative and halophilic bacterium with a single polar flagellum known as seafood-borne gastroenteritis throughout the world. These bacteria are characterized by three major clinical manifestations, including wound infection, primary septicemia and gastroenteritis. Accordingly, the present cross-sectional study aimed to detect V. parahaemolyticus using PCR method in 80 fresh shrimp samples collected from different seafood centers in Ardabil, Iran. The samples after immediately homogenization were cultured in TCBS agar. The resulting isolates were identified by biochemical tests, and then typical colonies were selected for DNA extraction. Next, the PCR technique was employed to detect the presence or absence of vp-toxR gene as a marker of these bacteria. Based on the findings, 11 (13. 75 %) out of 80 fresh shrimp samples were positive for V. parahaemolyticus. According to the results, shrimp could be the major sources of V. parahaemolyticus that have a potential risk to the public health inasmuch as might be viable and infectious.

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    APA: Copy

    Asgarpoor, d., GHASEMI, M., & BAHRAMI, M.. (2018). Detection of Vibrio parahaemolyticus in shrimp samples collected from Ardabil by PCR method. JOURNAL OF FOOD MICROBIOLOGY, 5(3 ), 26-35. SID. https://sid.ir/paper/250692/en

    Vancouver: Copy

    Asgarpoor d., GHASEMI M., BAHRAMI M.. Detection of Vibrio parahaemolyticus in shrimp samples collected from Ardabil by PCR method. JOURNAL OF FOOD MICROBIOLOGY[Internet]. 2018;5(3 ):26-35. Available from: https://sid.ir/paper/250692/en

    IEEE: Copy

    d. Asgarpoor, M. GHASEMI, and M. BAHRAMI, “Detection of Vibrio parahaemolyticus in shrimp samples collected from Ardabil by PCR method,” JOURNAL OF FOOD MICROBIOLOGY, vol. 5, no. 3 , pp. 26–35, 2018, [Online]. Available: https://sid.ir/paper/250692/en

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