EVALUATION OF VIRULENCE AND ENTEROTOXIN GENES IN SALMONELLA ENTERITIDIS STRAINS ISOLATED FROM MEAT AND EGG SAMPLES BY MULTIPLEX-PCR

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KHODADADIPOUR TOKTAM; AMINI KUMARSS; MAHMOUDI RAZZAGH

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Journal: JOURNAL OF FOOD MICROBIOLOGY Year:2016 | Volume:3 | Issue:2 Page(s): 25-33

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Title

EVALUATION OF VIRULENCE AND ENTEROTOXIN GENES IN SALMONELLA ENTERITIDIS STRAINS ISOLATED FROM MEAT AND EGG SAMPLES BY MULTIPLEX-PCR

Author(s)

KHODADADIPOUR TOKTAM | AMINI KUMARSS | MAHMOUDI RAZZAGH | Issue Writer Certificate

Keywords

ENTEROTOXIN GENEQ2

MULTIPLEX POLYMERASE CHAIN REACTIONQ1

SALMONELLA ENTERITIDISQ2

VIRULENCE GENEQ1

Abstract

Today, food borne diseases are one of the most serious problems and occur as a result of consumption to contaminated food and water. The aim of the study was evaluation of virulence and ENTEROTOXIN GENEs in SALMONELLA ENTERITIDIS strains isolated from food samples by multiplex- PCR. This descriptive, cross-sectional study was performed at 2014 on the 1250 no duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of Stn, sopB, slyA, spvc and Phop/Q genes. Sixty SALMONELLA ENTERITIDIS strains were obtained from poultry meat (35 strains, 58.3%) and eggs (25 strains, 41.6%), respectively. molecular analysis distribution showed all isolates (100%) were absence for spvC gene. The highest and lowest prevalence of the genes were related to Phop/Q and SopB, 33.3% and 1.6%, respectively. Evaluation of VIRULENCE GENEs and enterotoxin in the SALMONELLA ENTERITIDIS isolated from the food samples are useful because of presence of the genes and efficacy of M-PCR method in epidemiological investigation and assessment of intraspecies genes transfer in food samples.

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APA: Copy

KHODADADIPOUR, TOKTAM, AMINI, KUMARSS, & MAHMOUDI, RAZZAGH. (2016). EVALUATION OF VIRULENCE AND ENTEROTOXIN GENES IN SALMONELLA ENTERITIDIS STRAINS ISOLATED FROM MEAT AND EGG SAMPLES BY MULTIPLEX-PCR. JOURNAL OF FOOD MICROBIOLOGY, 3(2), 25-33. SID. https://sid.ir/paper/250772/en

Vancouver: Copy

KHODADADIPOUR TOKTAM, AMINI KUMARSS, MAHMOUDI RAZZAGH. EVALUATION OF VIRULENCE AND ENTEROTOXIN GENES IN SALMONELLA ENTERITIDIS STRAINS ISOLATED FROM MEAT AND EGG SAMPLES BY MULTIPLEX-PCR. JOURNAL OF FOOD MICROBIOLOGY[Internet]. 2016;3(2):25-33. Available from: https://sid.ir/paper/250772/en

IEEE: Copy

TOKTAM KHODADADIPOUR, KUMARSS AMINI, and RAZZAGH MAHMOUDI, “EVALUATION OF VIRULENCE AND ENTEROTOXIN GENES IN SALMONELLA ENTERITIDIS STRAINS ISOLATED FROM MEAT AND EGG SAMPLES BY MULTIPLEX-PCR,” JOURNAL OF FOOD MICROBIOLOGY, vol. 3, no. 2, pp. 25–33, 2016, [Online]. Available: https://sid.ir/paper/250772/en

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