CLONING OF BVDV NS3 AND BVDV 5'UTR INTO PWPI- LINKER B LENTIVIRAL PLASMID

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MOKHTARI A.; MADADGAR O.; GHALYANCHI LANGEROUDI A.; MAHZOUNIEH M.

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Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES Year:2017 | Volume:10 | Issue:2 Page(s): 13-24

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Title

CLONING OF BVDV NS3 AND BVDV 5'UTR INTO PWPI- LINKER B LENTIVIRAL PLASMID

Author(s)

MOKHTARI A. | MADADGAR O. | GHALYANCHI LANGEROUDI A. | MAHZOUNIEH M. | Issue Writer Certificate

Keywords

BVDVQ2

NS3Q1

5’UTRQ1

CLONINGQ1

PWPI- LINKER BQ1

Abstract

Bovine viral diarrhea virus (BVDV) is a genus Pestivirus of the family Flaviviridae and causes significant economic and hygienic losses in cattle farms throughout the world. The virus has spread worldwide and despite the wide use of various control strategies against it, BVD remains prevalent. In recent years, numerous measures such as anti-viral gene therapies are frequently employed to control BVDV infection. One way to evaluate these measures is the CLONING of conserved areas of the viral genome into suitable vectors for the preparation of cell lines expressing sub- genomic replicons of the virus. In this study, after multiplication of conserved sequences of BVDV- NADL NS3 and5’UTR by RT-PCR, these fragments were cloned in PWPI- LINKER B lentiviral vector at the upstream of GFP gene. The validity of CLONING was confirmed using restriction enzyme digestion and sequencing.Therefore these recombinant plasmids will be available to produce lentiviral transfer vectors carrying the transgenes using the lentiviral packaging systems.

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APA: Copy

MOKHTARI, A., MADADGAR, O., GHALYANCHI LANGEROUDI, A., & MAHZOUNIEH, M.. (2017). CLONING OF BVDV NS3 AND BVDV 5'UTR INTO PWPI- LINKER B LENTIVIRAL PLASMID. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, 10(2), 13-24. SID. https://sid.ir/paper/260091/en

Vancouver: Copy

MOKHTARI A., MADADGAR O., GHALYANCHI LANGEROUDI A., MAHZOUNIEH M.. CLONING OF BVDV NS3 AND BVDV 5'UTR INTO PWPI- LINKER B LENTIVIRAL PLASMID. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES[Internet]. 2017;10(2):13-24. Available from: https://sid.ir/paper/260091/en

IEEE: Copy

A. MOKHTARI, O. MADADGAR, A. GHALYANCHI LANGEROUDI, and M. MAHZOUNIEH, “CLONING OF BVDV NS3 AND BVDV 5'UTR INTO PWPI- LINKER B LENTIVIRAL PLASMID,” IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, vol. 10, no. 2, pp. 13–24, 2017, [Online]. Available: https://sid.ir/paper/260091/en

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