Cloning and expression of viral haemorrhagic septicaemia nucleocapsid (N) gene of Rainbow trout (Onchorhynchus mykiss) in Escherichia coli

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAHNAMA R.; PEYGHAN R.; SEYFIABAD SHAPOURI M.R.; REZAIE A.; SHAHBAZIAN N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES Year:2019 | Volume:12 | Issue:2 Page(s): 97-107

Download Full-Text

Persian Verion

View:

365

Download:

Cites:

Information Journal Paper

Title

Cloning and expression of viral haemorrhagic septicaemia nucleocapsid (N) gene of Rainbow trout (Onchorhynchus mykiss) in Escherichia coli

Author(s)

Keywords

Nucleocapsid Recombinant proteinQ1

viral hemorrhagic septicemia (VHS)Q1

Rainbow troutQ2

Abstract

Septicemia viral disease (VHS) is one of the most important diseases of fish that has recently been developed in Iran and has caused severe damage to breeders. In order to identify the possible cause of the losses, farmed fish were sampled from epicenter where massive mortalities and economic losses had been occurred. Samples were collected from relevant tissues (i. e. liver, kidney, spleen, heart, and brain) of Rainbow trout with clinical signs of VHS disease. The samples were stored in 70% ethanol, to perform RT-PCR. Then, recombinant plasmids were tested for protein expression in E. coli Rosetta strain. SDS-PAGE analysis indicated the production of a recombinant protein with an expected molecular weight of 61KDa. Thereafter, the recombinant protein was purified by amylose resin and its antigenicity was accessed by immunoblotting using a mouse polyclonal serum prepared against MBP protein. Positive reaction of the expressed protein with anti-MBP protein indicated that the expressed N protein possess antigenic epitopes and could be applied in future immunological studies.

Cites

No record.

References

No record.

Cite

APA: Copy

RAHNAMA, R., PEYGHAN, R., SEYFIABAD SHAPOURI, M.R., REZAIE, A., & SHAHBAZIAN, N.. (2019). Cloning and expression of viral haemorrhagic septicaemia nucleocapsid (N) gene of Rainbow trout (Onchorhynchus mykiss) in Escherichia coli. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, 12(2 ), 97-107. SID. https://sid.ir/paper/260097/en

Vancouver: Copy

RAHNAMA R., PEYGHAN R., SEYFIABAD SHAPOURI M.R., REZAIE A., SHAHBAZIAN N.. Cloning and expression of viral haemorrhagic septicaemia nucleocapsid (N) gene of Rainbow trout (Onchorhynchus mykiss) in Escherichia coli. IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES[Internet]. 2019;12(2 ):97-107. Available from: https://sid.ir/paper/260097/en

IEEE: Copy

R. RAHNAMA, R. PEYGHAN, M.R. SEYFIABAD SHAPOURI, A. REZAIE, and N. SHAHBAZIAN, “Cloning and expression of viral haemorrhagic septicaemia nucleocapsid (N) gene of Rainbow trout (Onchorhynchus mykiss) in Escherichia coli,” IRANIAN JOURNAL OF VETERINARY CLINICAL SCIENCES, vol. 12, no. 2 , pp. 97–107, 2019, [Online]. Available: https://sid.ir/paper/260097/en

Related Journal Papers