Optimization of Arabidopsis germination system for nutritional studies under soilless culture

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NAZARIDELJOU MOHAMMAD JAVAD; Delden Sander van; MARCELIS L.F.M.

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Journal: HORTICULTURAL PLANT NUTRITION Year:2019 | Volume:2 | Issue:1 Page(s): 21-32

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Title

Optimization of Arabidopsis germination system for nutritional studies under soilless culture

Author(s)

NAZARIDELJOU MOHAMMAD JAVAD | Delden Sander van | MARCELIS L.F.M. | Issue Writer Certificate

Keywords

Agar

germination medium

seedling growth

nutrition element

model plant

Abstract

Introduction: Arabidopsis thaliana (Arabidopsis thaliana) is the most important model plant in plant nutrition studies, genetics, and plant biotechnology. Arabidopsis cultivation is mainly done in either soil or soilless systems. Soil cultivation of Arabidopsis in compared to soilless culture does not have precise control on the concentration and ratio of mineral elements in the substrate, salinity, and pH, which results in negative effects on plant growth, biochemical reactions, etc. However, soilless or hydroponic systems due to the exact control of plant nutritional needs and the substrate are more compatible for high accuracy experiments such as plant nutrition, especially for primary seedling growth. Accordingly, this experiment was conducted to provide an optimized Arabidopsis germination system for production of healthy seedlings for soilless systems based on the Agar-nutrient media. Materials and Methods: This experiment was conducted based on the importance of the development of Arabidopsis in soilless systems in order to provide accurate nutritional and physiological trials and the importance of effective germination and the production of healthy seedlings in an Agar-nutrient media. Therefore, two milliliters microtubes were cut into 10 mm in length, and connected upsidedown to the table with adhesive tape then were filled with Agar (0. 55%) and mineral elements (Hoagland formulation) medium. (1: 1 V/V). Sterilized and stratified Arabidopsis seeds were sown on filled microtubes and then transferred to the growth chamber with controlled environmental conditions. Germination percentage and growth and development parameters of seedlings were measured for three weeks. Results and discussion: Results showed the effectiveness of the optimized germination system and also the Agar-nutrient medium for Arabidopsis. Accordingly, seed germination percentage was more than 97. 5%. Also, production of healthy seedlings with optimum growth (85%) especially the production of intact roots for various biological root and nutritional studies was another advantage of this experiment. Based on the results three weeks old plants were ready for transplanting to the main cultivation system. Conclusion: The results of the present study led to the conclusion that optimized system and medium due to high efficiency, simple and possible design, precise control of the substrate and root medium, could be recommended for Arabidopsis germination experiments and also arabidopsis cultivation for nutritional purposes.

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APA: Copy

NAZARIDELJOU, MOHAMMAD JAVAD, Delden, Sander van, & MARCELIS, L.F.M.. (2019). Optimization of Arabidopsis germination system for nutritional studies under soilless culture. HORTICULTURAL PLANT NUTRITION, 2(1 ), 21-32. SID. https://sid.ir/paper/268525/en

Vancouver: Copy

NAZARIDELJOU MOHAMMAD JAVAD, Delden Sander van, MARCELIS L.F.M.. Optimization of Arabidopsis germination system for nutritional studies under soilless culture. HORTICULTURAL PLANT NUTRITION[Internet]. 2019;2(1 ):21-32. Available from: https://sid.ir/paper/268525/en

IEEE: Copy

MOHAMMAD JAVAD NAZARIDELJOU, Sander van Delden, and L.F.M. MARCELIS, “Optimization of Arabidopsis germination system for nutritional studies under soilless culture,” HORTICULTURAL PLANT NUTRITION, vol. 2, no. 1 , pp. 21–32, 2019, [Online]. Available: https://sid.ir/paper/268525/en

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