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Information Journal Paper

Title

The effects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage

Pages

  51-59

Abstract

 Th is study examined the eff ects of skim-milk based extenders supplemented with proline and glutamine on motility, lipid peroxidation and enzymatic antioxidant status of cooled-stored equine sperm and determined the role of seminal plasma as well. Th e semen was collected with artifi cial vagina. In experiment 1, native semen was diluted in skim-milk based extender containing 5mM glutamine and 3mM proline, stored at 5° C and analyzed at 4, 24 and 48 hours storage for motion parameters. In experiment 2, semen was centrifugated, sperm pellet resuspended in the extenders and stored at 5° C for 4 hours to determine motion parameters. Th e level of catalase, glutathione peroxidase activity and malondialdehyde formation was determined for all samples at 4 hours. glutamine and proline signifi cantly preserved the percentage of motile sperm (76. 5 ± 2. 7 and 79. 4 ± 1 vs 69 ± 1. 4), increased the progressive motility of cold-stored semen (66. 1 ± 2. 5 and 73. 7 ± 2. 9 vs 56. 2 ± 1. 4), increased catalase activity, and decreased malondialdehyde. However these eff ects were disappeared after seminal plasma removal. We conclude that glutamine and proline would amplify the antioxidant activity of equine cold-stored semen and preserve its motility. Th is eff ect seems to be related to interactions with seminal plasma.

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    APA: Copy

    DAVOODIAN, NAJMEH, KADIVAR, ALI, & AHMADI, EBRAHIM. (2019). The effects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage. IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, 11(1 ), 51-59. SID. https://sid.ir/paper/269143/en

    Vancouver: Copy

    DAVOODIAN NAJMEH, KADIVAR ALI, AHMADI EBRAHIM. The effects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage. IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY[Internet]. 2019;11(1 ):51-59. Available from: https://sid.ir/paper/269143/en

    IEEE: Copy

    NAJMEH DAVOODIAN, ALI KADIVAR, and EBRAHIM AHMADI, “The effects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage,” IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, vol. 11, no. 1 , pp. 51–59, 2019, [Online]. Available: https://sid.ir/paper/269143/en

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