Serological and molecular studies on visceral leishmaniosis in stray dogs in Torbat-e-Heidareih area

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Pourali Mohsen; RAZMI GHOLAMREZA; Sabzavari Sadaf

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY Year:2017 | Volume:9 | Issue:1 Page(s): 7-11

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Title

Serological and molecular studies on visceral leishmaniosis in stray dogs in Torbat-e-Heidareih area

Author(s)

Pourali Mohsen | RAZMI GHOLAMREZA | Sabzavari Sadaf | Issue Writer Certificate

Keywords

Leishmania infantum

stray dogs

Torbat-e-Heidareih area

Abstract

Visceral leishmaniosis is an important zoonotic disease in Iran. Th e agent, vector and reservoir are Leishmania infantum, Phlebetomous major and dogs, respectively. Th us far, no data has been reported about the prevalence of visceral leishmaniosis in human and dog in Torbat-e-Heidareah area and the present study has been performed to determine the frequency of L. infantum infection in stray dogs. One hundred blood and skin samples were collected from 2014 to 2015. Th e sera samples were examined by Indirect Immunofl uorescent Antibody Test (IFAT). Th e skin samples of seropositive and seronegative in association with skin lesions were examined by PCR method. In this study, antibodies against L. infantum were de-tected in 3 (3%) sera samples of dogs and kDNA Leishmania spp. was detected in 4 (4%) of seronegative dogs by PCR. L. infantum was detected in positive samples by semi nested PCR. Based on serological and molecular results, 7% of asymptomatic stray dogs of Torbat-e-Heidareih area were infected with L. infantum and may be acted as reservoirs of visceral leishmaniosis.

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APA: Copy

Pourali, Mohsen, RAZMI, GHOLAMREZA, & Sabzavari, Sadaf. (2017). Serological and molecular studies on visceral leishmaniosis in stray dogs in Torbat-e-Heidareih area. IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, 9(1 ), 7-11. SID. https://sid.ir/paper/269155/en

Vancouver: Copy

Pourali Mohsen, RAZMI GHOLAMREZA, Sabzavari Sadaf. Serological and molecular studies on visceral leishmaniosis in stray dogs in Torbat-e-Heidareih area. IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY[Internet]. 2017;9(1 ):7-11. Available from: https://sid.ir/paper/269155/en

IEEE: Copy

Mohsen Pourali, GHOLAMREZA RAZMI, and Sadaf Sabzavari, “Serological and molecular studies on visceral leishmaniosis in stray dogs in Torbat-e-Heidareih area,” IRANIAN JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, vol. 9, no. 1 , pp. 7–11, 2017, [Online]. Available: https://sid.ir/paper/269155/en

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