Identification and Cloning of a Cotton Appropriate Reference Gene for Increasing Efficiency of Molecular Analysis

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TOHIDFAR MASOUD; DEZHSETAN SARA

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Paper Information

Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2017 | Volume:6 | Issue:1 Page(s): 77-84

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Information Journal Paper

Title

Identification and Cloning of a Cotton Appropriate Reference Gene for Increasing Efficiency of Molecular Analysis

Author(s)

TOHIDFAR MASOUD | DEZHSETAN SARA | Issue Writer Certificate

Keywords

Bioinformatics

Cloning

Cotton

Reference gene

Sad gene

Abstract

An appropriate Reference gene for assessment of DNA genome quality and analysis of transgenic crops is very important. In this research, we have evaluated the SadI gene as a potential Reference gene in Cotton. At the first, DNA was extracted, and then a PCR reaction was done using appropriative primers designed for Vector NTI. The PCR products were and bp fragments. The fragments were cloned in pTZ R/T plasmid. Recombinant plasmids were identified by colony PCR with appropriative primers and M primers and then were sequenced. The sequencing results showed that these two fragments are related to two different copies of the SadI gene that in one of copies a bp deletion had occurred in the second intron. This research is the first report from sequencing of a Cotton Reference gene that showing the presence of two different copies of SadI gene, and confirmed that it is a suitable Reference gene for different studies

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APA: Copy

TOHIDFAR, MASOUD, & DEZHSETAN, SARA. (2017). Identification and Cloning of a Cotton Appropriate Reference Gene for Increasing Efficiency of Molecular Analysis. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 6(1 ), 77-84. SID. https://sid.ir/paper/270517/en

Vancouver: Copy

TOHIDFAR MASOUD, DEZHSETAN SARA. Identification and Cloning of a Cotton Appropriate Reference Gene for Increasing Efficiency of Molecular Analysis. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2017;6(1 ):77-84. Available from: https://sid.ir/paper/270517/en

IEEE: Copy

MASOUD TOHIDFAR, and SARA DEZHSETAN, “Identification and Cloning of a Cotton Appropriate Reference Gene for Increasing Efficiency of Molecular Analysis,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 6, no. 1 , pp. 77–84, 2017, [Online]. Available: https://sid.ir/paper/270517/en

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