CLONING OF CHOLESTEROL OXIDASE GENE FROM IRANIAN STRAIN OF RHODOCOCCUS FOR PRODUCTION OF COTTON BIOLOGICAL PESTICIDE

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BAHRAMI SABA; TOHIDFAR MASOOD; EBRAHIMI MOHAMMAD ALI; ESMAEIL LASHGARIAN HAMED

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Journal: IRANIAN JOURNAL OF COTTON RESEARCHES Year:2016 | Volume:3 | Issue:2 Page(s): 55-64

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Information Journal Paper

Title

CLONING OF CHOLESTEROL OXIDASE GENE FROM IRANIAN STRAIN OF RHODOCOCCUS FOR PRODUCTION OF COTTON BIOLOGICAL PESTICIDE

Author(s)

BAHRAMI SABA | TOHIDFAR MASOOD | EBRAHIMI MOHAMMAD ALI | ESMAEIL LASHGARIAN HAMED | Issue Writer Certificate

Keywords

CHOLESTEROL OXIDASE

CLONING

PET23A

COTTON BIOLOGICAL PESTICIDES

Abstract

CHOLESTEROL OXIDASE enzyme, as a new generation of biological pesticides, has pesticides strong effect on the pest to cotton Heliothis armigera. The mechanism of action of CHOLESTEROL OXIDASE fatality related to the oxidation of cholesterol in the membranes of insect midgut epithelial which leads to physical destruction and the resulting disturbances in membrane structure and function and eventually death of the insect. In order to retrofitting genetically engineered plants against pests, production of them with the gene coding for CHOLESTEROL OXIDASE enzyme, require the best method for expression level and activity of this enzyme. Therefore, this study aimed to clone CHOLESTEROL OXIDASE enzyme in plasmid PET23A for possible guidance recombinant protein produced in bacterial periplasmic space. Rhodococcus sp. strain 502 that produce high level of CHOLESTEROL OXIDASE, was used for gene isolation by PCR. The PCR product was sub cloned in plasmid pJET, subsequently for expression was cloned in PET23A, eventually was transformed into the host E.coli DH5a. Recombinant plasmid PET23A CHO was confirmed by restriction enzyme digestion and sequencing. The results of sequencing showed high similarity between our isolated gene sequence and the other CHOLESTEROL OXIDASE gene sequences in NCBI that can be used for production of biological pesticide.

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APA: Copy

BAHRAMI, SABA, TOHIDFAR, MASOOD, EBRAHIMI, MOHAMMAD ALI, & ESMAEIL LASHGARIAN, HAMED. (2016). CLONING OF CHOLESTEROL OXIDASE GENE FROM IRANIAN STRAIN OF RHODOCOCCUS FOR PRODUCTION OF COTTON BIOLOGICAL PESTICIDE. IRANIAN JOURNAL OF COTTON RESEARCHES, 3(2 ), 55-64. SID. https://sid.ir/paper/266172/en

Vancouver: Copy

BAHRAMI SABA, TOHIDFAR MASOOD, EBRAHIMI MOHAMMAD ALI, ESMAEIL LASHGARIAN HAMED. CLONING OF CHOLESTEROL OXIDASE GENE FROM IRANIAN STRAIN OF RHODOCOCCUS FOR PRODUCTION OF COTTON BIOLOGICAL PESTICIDE. IRANIAN JOURNAL OF COTTON RESEARCHES[Internet]. 2016;3(2 ):55-64. Available from: https://sid.ir/paper/266172/en

IEEE: Copy

SABA BAHRAMI, MASOOD TOHIDFAR, MOHAMMAD ALI EBRAHIMI, and HAMED ESMAEIL LASHGARIAN, “CLONING OF CHOLESTEROL OXIDASE GENE FROM IRANIAN STRAIN OF RHODOCOCCUS FOR PRODUCTION OF COTTON BIOLOGICAL PESTICIDE,” IRANIAN JOURNAL OF COTTON RESEARCHES, vol. 3, no. 2 , pp. 55–64, 2016, [Online]. Available: https://sid.ir/paper/266172/en

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