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Information Journal Paper

Title

Inhibition of the boll worm, Helicoverpa armigera amyloeletic activity by seed protein extracts of Prosopis farcta and Cicer arietinum

Pages

  333-341

Abstract

 Cotton Boll worm, Helicoverpa armigera (Hü bner) (Lepidoptera: Noctuidae) is an important polyphagous pest throughout the world. Due to the adverse effects of chemical pesticides, alpha-amylase enzyme inhibitors in insects have been noticed as an alternative method for the pest management programs. In this project, whole gut of the fourth instar larva was dissected and the enzyme extract was pulled out. After removing seed protein extracts of Prosopis farcta and Cicer arietinum by sodium chloride 0. 1 M, the effect of four different concentrations of both seed extracts (1. 48, 1. 2, 0. 87 and 0. 42 μ g) were studied on alpha-amylase activity of Boll worm. According to the data, the protein extract of P. farcta had greater ability to inhibit cotton Boll worm amylase. Enzyme assays in the presence of different concentrations of C. arietinum and P. farcta extracts showed enzyme inhibition in a dose-dependent manner. The maximum enzyme inhibition was in pH 9. Zymogram analysis confirmed quantitative enzyme assay and showed a sharp reduction in the band thickness during the amylase inhibition. Consequently, the seeds of both P. farcta and C. arietinum, had the potential to be considered as effective and important sources of alpha-amylase inhibitors and noticed as substitutes for chemical pesticides in the near future.

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  • Cite

    APA: Copy

    Azimi, Solmaz, RAHMANI, SHIMA, & Majidianii, Sima. (2018). Inhibition of the boll worm, Helicoverpa armigera amyloeletic activity by seed protein extracts of Prosopis farcta and Cicer arietinum. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 6(2 ), 333-341. SID. https://sid.ir/paper/270533/en

    Vancouver: Copy

    Azimi Solmaz, RAHMANI SHIMA, Majidianii Sima. Inhibition of the boll worm, Helicoverpa armigera amyloeletic activity by seed protein extracts of Prosopis farcta and Cicer arietinum. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2018;6(2 ):333-341. Available from: https://sid.ir/paper/270533/en

    IEEE: Copy

    Solmaz Azimi, SHIMA RAHMANI, and Sima Majidianii, “Inhibition of the boll worm, Helicoverpa armigera amyloeletic activity by seed protein extracts of Prosopis farcta and Cicer arietinum,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 6, no. 2 , pp. 333–341, 2018, [Online]. Available: https://sid.ir/paper/270533/en

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