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Information Journal Paper

Title

Bread and Durum Wheat Proteome Response to Wheat Fusarium Head Blight

Author(s)

Sheikhhesabi Tayebeh | Jahanbakhsh Godehkahriz Soodabeh | DAVARI MAHDI | Issue Writer Certificate 

Pages

  25-37

Abstract

 Fusarium Head blight (FHB), caused by Fusarium graminearum known as a destructive disease of wheat has a significant impact not only on global wheat production, but also on food safety because of grain contamination with mycotoxins during infection, a serious threat to the human and animals’ health. To achieve to non-chemical control of FHB, the study of molecular mechanism of Resistance to FHB in wheat varieties is important. In this research, in order to investigate the effect of FHB on spike proteome pattern, six bread and durum wheat cultivars were inoculated in a greenhouse with F. graminearum. In addition to measuring the morphological traits, 14 days after inoculation, changes in their proteome pattern were investigated. Two-dimensional electrophoresis technique was used to study the response of wheat spike to Fusarium. Protein extraction was performed by TCAacetone method and two-dimensional electrophoresis was performed in the first dimension by strip gel method and in the second dimension was performed by using sodium dodecyl sulfate polyacrylamide gel. The results of gel analyses showed that out of 57 repeatedly found Protein spots, 13 Protein spots had a significant difference between treatments, among which 11 Protein spots, showed increased expression and two Protein spots showed reduced expression. Proteins with changes in expression based on biological function were categorized in different groups that Proteins involved in the metabolic pathways which involved in infected plants (Hypothetical Protein and PR Proteins) had the highest contribution to Proteins with significant changes. The results of this study can be used in wheat breeding programs.

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  • Cite

    APA: Copy

    Sheikhhesabi, Tayebeh, Jahanbakhsh Godehkahriz, Soodabeh, & DAVARI, MAHDI. (2019). Bread and Durum Wheat Proteome Response to Wheat Fusarium Head Blight. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 8(1 ), 25-37. SID. https://sid.ir/paper/270553/en

    Vancouver: Copy

    Sheikhhesabi Tayebeh, Jahanbakhsh Godehkahriz Soodabeh, DAVARI MAHDI. Bread and Durum Wheat Proteome Response to Wheat Fusarium Head Blight. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2019;8(1 ):25-37. Available from: https://sid.ir/paper/270553/en

    IEEE: Copy

    Tayebeh Sheikhhesabi, Soodabeh Jahanbakhsh Godehkahriz, and MAHDI DAVARI, “Bread and Durum Wheat Proteome Response to Wheat Fusarium Head Blight,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 8, no. 1 , pp. 25–37, 2019, [Online]. Available: https://sid.ir/paper/270553/en

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