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Information Journal Paper

Title

SCREENING OF SOME SOIL FUSARIA FOR CELLULOSE ACTIVITY AND PARTIAL PURIFICATION OF CELLULOSE

Pages

  123-132

Keywords

CARBOXY METHYL CELLULOSE (CMC) 
WHEAT STRAW (WS) 

Abstract

 Several soil borne Fusaria species were screened for CELLULASE ACTIVITY, PARTIAL PURIFICATION and characterization of cellulase from superior isolates. Carboxy Methyl Cellulose (CMC) and Wheat Straw (WS) were used as two sole carbon sources separately in a minimal culture medium. RELEASED PROTEINs and sugars were assayed three days after inoculation with their related reagents and then these actions were repeated three days intervals. Statistical analysis among tested FUSARIUM species showed significant variation in RELEASED SUGARs, but no significant variation in RELEASED PROTEINs. The highest and lowest RELEASED SUGARs were produced by F. solani and F. oxysporium, respectively. The highest RELEASED SUGARs in F. solani isolates were observed 9 and 12 days post inoculation for WS and CMC media, respectively. RELEASED PROTEINs in F. solani indicated the highest increased in 12 and 6 days post inoculation on WS and CMC media, respectively. Optimal conditions for cellulase PARTIAL PURIFICATION of F. solani grown in CMC and WS supplemented media were pH 6 and temperature between 40-50oC. The study on protein bands showed that, only one with molecular weight of 24 kDa, out of all tested protein bands on CMC medium, indicated the CELLULASE ACTIVITY. Our observations showed that different Fusaria species have dissimilar behaviors and variable speeds in cellulose degradation. Also, WS medium showed high ability for producing cellulase enzyme that can be effectively used as a cheap organic waste medium.Characterization of cellulase F. solani showed that this strain produced a acidophilic, and thermostable cellulase.

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    Cite

    APA: Copy

    LOTFI, A., TAJICK GHANBARY, M.A., RANJBAR, G., & ASGHARZADEH, A.. (2011). SCREENING OF SOME SOIL FUSARIA FOR CELLULOSE ACTIVITY AND PARTIAL PURIFICATION OF CELLULOSE. JOURNAL OF BIODIVERSITY AND ECOLOGICAL SCIENCES (JBES), 1(2), 123-132. SID. https://sid.ir/paper/325727/en

    Vancouver: Copy

    LOTFI A., TAJICK GHANBARY M.A., RANJBAR G., ASGHARZADEH A.. SCREENING OF SOME SOIL FUSARIA FOR CELLULOSE ACTIVITY AND PARTIAL PURIFICATION OF CELLULOSE. JOURNAL OF BIODIVERSITY AND ECOLOGICAL SCIENCES (JBES)[Internet]. 2011;1(2):123-132. Available from: https://sid.ir/paper/325727/en

    IEEE: Copy

    A. LOTFI, M.A. TAJICK GHANBARY, G. RANJBAR, and A. ASGHARZADEH, “SCREENING OF SOME SOIL FUSARIA FOR CELLULOSE ACTIVITY AND PARTIAL PURIFICATION OF CELLULOSE,” JOURNAL OF BIODIVERSITY AND ECOLOGICAL SCIENCES (JBES), vol. 1, no. 2, pp. 123–132, 2011, [Online]. Available: https://sid.ir/paper/325727/en

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