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Information Journal Paper

Title

OPTIMIZATION OF IN VITRO PROPAGATION OF QARE-QAT (VACCINIUM ARCTOSTAPHYLUS)

Pages

  199-205

Abstract

 In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared.Thein vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7 g L-1), or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage AN media supplemented with 4 mg L-1 zeatin (pH=5.5) and 1 mg L-1 zeatin (pH=5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA.The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing of 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively.The plantlets were successfully transferred to soil and the survival rate was 85%.

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    APA: Copy

    HASANLOO, TAHEREH, JAFARKHANI KERMANI, MARYAM, MALMIR CHEGINI, MAHSA, SEPEHRIFAR, ROSHANAK, MOHAJERI NARAGHI, SEPEHR, & MIRI, SEIED MEHDI. (2014). OPTIMIZATION OF IN VITRO PROPAGATION OF QARE-QAT (VACCINIUM ARCTOSTAPHYLUS). JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS, 3(2), 199-205. SID. https://sid.ir/paper/331490/en

    Vancouver: Copy

    HASANLOO TAHEREH, JAFARKHANI KERMANI MARYAM, MALMIR CHEGINI MAHSA, SEPEHRIFAR ROSHANAK, MOHAJERI NARAGHI SEPEHR, MIRI SEIED MEHDI. OPTIMIZATION OF IN VITRO PROPAGATION OF QARE-QAT (VACCINIUM ARCTOSTAPHYLUS). JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS[Internet]. 2014;3(2):199-205. Available from: https://sid.ir/paper/331490/en

    IEEE: Copy

    TAHEREH HASANLOO, MARYAM JAFARKHANI KERMANI, MAHSA MALMIR CHEGINI, ROSHANAK SEPEHRIFAR, SEPEHR MOHAJERI NARAGHI, and SEIED MEHDI MIRI, “OPTIMIZATION OF IN VITRO PROPAGATION OF QARE-QAT (VACCINIUM ARCTOSTAPHYLUS),” JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS, vol. 3, no. 2, pp. 199–205, 2014, [Online]. Available: https://sid.ir/paper/331490/en

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