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Information Journal Paper

Title

ALLOGENIC VS. SYNTHETIC GRANULES FOR BONE TISSUE ENGINEERING: AN IN VITRO STUDY

Pages

  133-141

Abstract

 The aim of this study was to compare human dental pulp stem cells’ (DPSCs) attachment, proliferation and osteogenic differentiationon allogenic and synthetic biphasic bone granules. In this IN VITRO STUDY, two types of bone granules were used: allograft [freeze-dried bone allograft (FDBA)] and biphasic granules [hydroxyapatite/beta-tricalcium phosphate (HA/β-TCP)]. By isolation of DPSCs, their attachment to bone granules was observed by scanning electron microscope (SEM) at day1 and 7 of cultivation. Vital cells were measured by MTT assay at 1, 3, and 7 days of cell culture. Comparison of vital cellsat different time points was considered as cell proliferation. Finally, differentiation of DPSCs was evaluated by measurementof alkaline phosphatase (ALP) activity 3, 7, 14, and 21 days after cell seeding in standard and osteogenic media. Data wereanalyzed using two-way ANOVA with a significant level of 0. 05. Attachment of DPSCs on FDBA granules seemed relativelystronger. The number of cells (based on MTT values) and ALP activity of the cells cultured on both study groups increasedbetween time points (p ≤ 0. 001). FDBA granules had more cells compared to HA/β-TCP granules (p < 0. 001). There was nosignificant difference between ALP activity of two study groups cultured in the standard medium (p = 0. 347) and they wereboth higher than the control group (p < 0. 05). In the osteogenic medium, FDBA group had significantly higher ALP activitycompared to HA/β-TCP (p = 0. 035) and control (p = 0. 001) groups while there was no significant difference between ALPactivity of HA/β-TCP and control groups (p = 0. 645). In conclusion, current IN VITRO STUDY revealed that FDBA granules havemore potential in supporting DPSCs attachment and proliferation and inducing their ALP activity compared to HA/β-TCPgranules. Therefore, FDBA could serve as a proper bone substitute material.

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    • References

    Cite

    APA: Copy

    KOUHESTANI, FARNAZ, DEHABADI, FARNAZ, HASAN SHAHRIARI, MEHRNOOSH, & MOTAMEDIAN, SAEED REZA. (2018). ALLOGENIC VS. SYNTHETIC GRANULES FOR BONE TISSUE ENGINEERING: AN IN VITRO STUDY. PROGRESS IN BIOMATERIALS, 7(2), 133-141. SID. https://sid.ir/paper/336529/en

    Vancouver: Copy

    KOUHESTANI FARNAZ, DEHABADI FARNAZ, HASAN SHAHRIARI MEHRNOOSH, MOTAMEDIAN SAEED REZA. ALLOGENIC VS. SYNTHETIC GRANULES FOR BONE TISSUE ENGINEERING: AN IN VITRO STUDY. PROGRESS IN BIOMATERIALS[Internet]. 2018;7(2):133-141. Available from: https://sid.ir/paper/336529/en

    IEEE: Copy

    FARNAZ KOUHESTANI, FARNAZ DEHABADI, MEHRNOOSH HASAN SHAHRIARI, and SAEED REZA MOTAMEDIAN, “ALLOGENIC VS. SYNTHETIC GRANULES FOR BONE TISSUE ENGINEERING: AN IN VITRO STUDY,” PROGRESS IN BIOMATERIALS, vol. 7, no. 2, pp. 133–141, 2018, [Online]. Available: https://sid.ir/paper/336529/en

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