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Information Journal Paper

Title

STRESS PHASE ANGLE REGULATES DIFFERENTIATION OF HUMAN ADIPOSE‑ DERIVEDSTEM CELLS TOWARD ENDOTHELIAL PHENOTYPE

Pages

  121-131

Abstract

 Endothelial cells are subjected to cyclic shear by pulsatile blood flow and pressures due to circumferential stresses. Althoughmost of the researches on this topic have considered the effects of these two biomechanical forces separately or concurrently, few studies have noticed the interaction of these cyclic loadings on endothelial behavior. Negative temporal stressphase angle, defined by the phase lag between cyclic shear and tensile stresses, is an established parameter which is knownto have substantial effects on blood vessel remodeling and progression of some serious cardiovascular diseases. In thisresearch, intermittent shear and tensile stresses with different stress phase angle values were applied on human adipose stemcells (ASC). The expression level of three major endothelial-specific genes, elastic modulus of cells and cytoskeleton actinstructure of cells were studied and compared among control and three test groups subjected to stress phase angle values at0° , − 45° , and − 90° . Mechanical properties of ASCs were determined by atomic force microscopy and actin fiber structurewas visualized by confocal imaging through Phalloidin staining. Results described a decrease in expression of FLK-1 andVE-cadherin and rise of vWF marker expression in case of higher negative stress phase angles. The Young’ s moduli ofcells were significantly higher and cytoskeletal actin structure was more organized with higher thickness for all test samplessubjected to combined stresses; however, these features were less magnificent for applied stress phase angles with highernegative values. The results confirmed significant effects of SPA on endothelial differentiation of mesenchymal stem cells.

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    Cite

    APA: Copy

    SHOJAEI, SHAHROKH, Tafazzoli Shadpour, Mohammad, SHOKRGOZAR, MOHAMMAD ALI, HAGHIGHIPOUR, NOOSHIN, & HEJAZI JAHROMI, FATEMEH. (2018). STRESS PHASE ANGLE REGULATES DIFFERENTIATION OF HUMAN ADIPOSE‑ DERIVEDSTEM CELLS TOWARD ENDOTHELIAL PHENOTYPE. PROGRESS IN BIOMATERIALS, 7(2), 121-131. SID. https://sid.ir/paper/336530/en

    Vancouver: Copy

    SHOJAEI SHAHROKH, Tafazzoli Shadpour Mohammad, SHOKRGOZAR MOHAMMAD ALI, HAGHIGHIPOUR NOOSHIN, HEJAZI JAHROMI FATEMEH. STRESS PHASE ANGLE REGULATES DIFFERENTIATION OF HUMAN ADIPOSE‑ DERIVEDSTEM CELLS TOWARD ENDOTHELIAL PHENOTYPE. PROGRESS IN BIOMATERIALS[Internet]. 2018;7(2):121-131. Available from: https://sid.ir/paper/336530/en

    IEEE: Copy

    SHAHROKH SHOJAEI, Mohammad Tafazzoli Shadpour, MOHAMMAD ALI SHOKRGOZAR, NOOSHIN HAGHIGHIPOUR, and FATEMEH HEJAZI JAHROMI, “STRESS PHASE ANGLE REGULATES DIFFERENTIATION OF HUMAN ADIPOSE‑ DERIVEDSTEM CELLS TOWARD ENDOTHELIAL PHENOTYPE,” PROGRESS IN BIOMATERIALS, vol. 7, no. 2, pp. 121–131, 2018, [Online]. Available: https://sid.ir/paper/336530/en

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