EVALUATION OF A DOT ELISA SYSTEM FOR DETECTION OF SEROCONVERSION TO INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS

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HEMATZADEH F.

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ISSN: 2588-4824

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Journal: Journal of Veterinary Research Year:2003 | Volume:58 | Issue:4 Page(s): 383-387

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Title

EVALUATION OF A DOT ELISA SYSTEM FOR DETECTION OF SEROCONVERSION TO INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS

Author(s)

HEMATZADEH F. | Issue Writer Certificate

Keywords

DOT ELISAQ4

ELISAQ4

IBRQ2

SEROLOGYQ4

HERPESVIRUSQ4

ANTIGENQ3

ANTIBODYQ3

PEROXIDASEQ3

Abstract

Objective: The purpose of this study was to designing a simple, sensitive and cheep method based on ELISA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">DOT ELISA system for detecting of seroconversion to infectious bovine rhinotracheitis virus (IBRV). Procedure: In the first step rabbit antibovine immunoglobins was conjugated with horseradish peroxidas and purifide IBR virus was used as coated ANTIGEN on nitrocellulose membrane. In order to standardization of the kit, 50 positive and 50 negative sera that tested by commercial ELISA kit (Svanova) and evaluated the best dilution o{the materials that used in the test. Results: The optimum dilution of different compound of the ELISA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">DOT ELISA kit Was: 1:200 for HRPO conjugated antiglobulin, 1:8 for serum and 1 for tetramethylbenzidin as substrate. In order to evaluation of correlation of ELISA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">DOT ELISA method with commercial ELISA kits, 488 serum samples were tested by two mentioned testes. Statistical analysis: Chi squire test. Conclusion: ELISA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">DOT ELISA has revealed 88/9% correlation with plate ELISA and no significant difference between results of these two tests.

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References

DEVELOPMENT OF AN IMMUNOFLUORESCENT KIT FOR IBR SERODIAGNOSIS

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APA: Copy

HEMATZADEH, F.. (2003). EVALUATION OF A DOT ELISA SYSTEM FOR DETECTION OF SEROCONVERSION TO INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS. JOURNAL OF VETERINARY RESEARCH, 58(4), 383-387. SID. https://sid.ir/paper/34533/en

Vancouver: Copy

HEMATZADEH F.. EVALUATION OF A DOT ELISA SYSTEM FOR DETECTION OF SEROCONVERSION TO INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS. JOURNAL OF VETERINARY RESEARCH[Internet]. 2003;58(4):383-387. Available from: https://sid.ir/paper/34533/en

IEEE: Copy

F. HEMATZADEH, “EVALUATION OF A DOT ELISA SYSTEM FOR DETECTION OF SEROCONVERSION TO INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS,” JOURNAL OF VETERINARY RESEARCH, vol. 58, no. 4, pp. 383–387, 2003, [Online]. Available: https://sid.ir/paper/34533/en

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