Allelic Descrimination of Canine MHC (DLA-DRB1) by High Resolution Melt Analysis

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VAHEDI S.M.; JAMSHIDI SH.; Lankarani Mohajer l.; NIKBAKHT BRUJENI GH.

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Journal: Journal of Veterinary Research Year:2018 | Volume:73 | Issue:1 Page(s): 93-100

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Title

Allelic Descrimination of Canine MHC (DLA-DRB1) by High Resolution Melt Analysis

Author(s)

VAHEDI S.M. | JAMSHIDI SH. | Lankarani Mohajer l. | NIKBAKHT BRUJENI GH. | Issue Writer Certificate

Keywords

MHCQ1

dogQ2

DLA-DRB1Q1

HRMQ1

Abstract

BACKGROUND: The function of molecules of major histocompatibility complex (MHC) is to bind antigenic peptides and display them on the cell surface for recognition by T cells. MHC polymorphism is related to suseptibility or resistance to immune-mediated and infectious diseases. High Resolution Melt (HRM) analysis is a novel and powerful technique in molecular biology for the detection of genetic polymorphisms and can be used for discrimination of MHC alleles and genotyping. OBJECTIVES: Discrimination of dog MHC alleles (DLA-DRB1) by high-resolution melt analysis. METHODS: Forty blood samples were collected from dogs referred to Small Animal Teaching Hospital of faculty of veterinary medicine, University of Tehran. After DNA extraction and amplification the exon 2 of DLA-DRB1, HRM and Melt Curve analysis was carried out. Direct sequencing was used for checking the HRM patterns. RESULTS: Following the HRM and Melt curve analysis, forty samples could be classified into 8 genotypes (A to H). Most frequent was A (25. 00%) and then C and E (each one 15. 00%). In total, 82. 5% and 17. 5% of samples were heterozygote and homozygote, respectively. CONCLUSIONS: In this study, the HRM technique was used for DLA-DRB1 gene alleles typing for the first time. Confirmation HRM results through sequencing results revealed that HRM can be used for discrimination of DLA-DRB1 alleles.

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APA: Copy

VAHEDI, S.M., JAMSHIDI, SH., Lankarani Mohajer, l., & NIKBAKHT BRUJENI, GH.. (2018). Allelic Descrimination of Canine MHC (DLA-DRB1) by High Resolution Melt Analysis. JOURNAL OF VETERINARY RESEARCH, 73(1 ), 93-100. SID. https://sid.ir/paper/34572/en

Vancouver: Copy

VAHEDI S.M., JAMSHIDI SH., Lankarani Mohajer l., NIKBAKHT BRUJENI GH.. Allelic Descrimination of Canine MHC (DLA-DRB1) by High Resolution Melt Analysis. JOURNAL OF VETERINARY RESEARCH[Internet]. 2018;73(1 ):93-100. Available from: https://sid.ir/paper/34572/en

IEEE: Copy

S.M. VAHEDI, SH. JAMSHIDI, l. Lankarani Mohajer, and GH. NIKBAKHT BRUJENI, “Allelic Descrimination of Canine MHC (DLA-DRB1) by High Resolution Melt Analysis,” JOURNAL OF VETERINARY RESEARCH, vol. 73, no. 1 , pp. 93–100, 2018, [Online]. Available: https://sid.ir/paper/34572/en

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