A Survey of Equine Herpes Virus 4 Infection in Four Provinces of Iran Using Real Time PCR Taqman Assay

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Tazikeh Arash; Raoofi Afshin; MADADGAR OMID; AKBAREIN HESAMEDDIN; GHADRDAN MASHHADI ALIREZA

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Journal: Journal of Veterinary Research Year:2018 | Volume:73 | Issue:4 Page(s): 483-490

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Title

A Survey of Equine Herpes Virus 4 Infection in Four Provinces of Iran Using Real Time PCR Taqman Assay

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Keywords

EquineQ3

Herpes virus 4Q1

Real Time PCRQ1

TaqmanQ1

Abstract

BACKGROUND: Herpes virus infections are pervasive in Equine populations worldwide, among them Equine Herpes virus 4 (EHV-4) is one of the major causes of economic losses in Equine industry. Infection with Equine Herpes virus 4 (EHV4) is well recognized as a cause of respiratory disease. OBJECTIVES: Evaluation of prevalence of the EHV4 in horses with clinical signs or history associated with these viruses from four provinces of Iran using the most sensitive method, Real Time PCR Taqman assay. METHODES: Blood samples and nasal swabs were taken from 150 horses with clinical signs or history associated with these viruses from four provinces (Tehran, Golestan, West Azarbaijan and Khuzestan) of Iran. DNA of samples extracted were then used for detection by Real Time PCR. RESULTS: From 150 sampled horses, a total of 18 (12%) were found to be positive for EHV-4. A total of 7 (4. 6%) blood samples and 11 (7. 3%) nasal swab samples were found to be positive for EHV-4. Out of 47 sampled horses from Golestan province, 45 sampled horses from Khuzestan province, 37 sampled horses from Tehran province and 21 sampled horses from West Azarbaijan, 6 (12. 76%), 3 (6. 6%), 5 (13. 5%) and 4 (19. 04%) were positive for EHV-4, respectively. CONCLUSIONS: This survey confirms the presence of EHV-4 in these provinces of Iran thus consideration should be given to preventive and control programs for dissemination of these viruses.

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APA: Copy

Tazikeh, Arash, Raoofi, Afshin, MADADGAR, OMID, AKBAREIN, HESAMEDDIN, & GHADRDAN MASHHADI, ALIREZA. (2018). A Survey of Equine Herpes Virus 4 Infection in Four Provinces of Iran Using Real Time PCR Taqman Assay. JOURNAL OF VETERINARY RESEARCH, 73(4 ), 483-490. SID. https://sid.ir/paper/34801/en

Vancouver: Copy

Tazikeh Arash, Raoofi Afshin, MADADGAR OMID, AKBAREIN HESAMEDDIN, GHADRDAN MASHHADI ALIREZA. A Survey of Equine Herpes Virus 4 Infection in Four Provinces of Iran Using Real Time PCR Taqman Assay. JOURNAL OF VETERINARY RESEARCH[Internet]. 2018;73(4 ):483-490. Available from: https://sid.ir/paper/34801/en

IEEE: Copy

Arash Tazikeh, Afshin Raoofi, OMID MADADGAR, HESAMEDDIN AKBAREIN, and ALIREZA GHADRDAN MASHHADI, “A Survey of Equine Herpes Virus 4 Infection in Four Provinces of Iran Using Real Time PCR Taqman Assay,” JOURNAL OF VETERINARY RESEARCH, vol. 73, no. 4 , pp. 483–490, 2018, [Online]. Available: https://sid.ir/paper/34801/en

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