A STUDY ON CELLULAR CHANGES IN INOCULATED CELL CULTURES WITH PESTIVIRUS, USING DIRECT IMMUNOFLURESCENCE TECHNIQUE

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HEMATZADEH F.; KEYVANFAR H.; BADAVAM M.; TAHERI MOHAMMAD

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Journal: Journal of Veterinary Research Year:2004 | Volume:59 | Issue:2 Page(s): 176-178

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Information Journal Paper

Title

A STUDY ON CELLULAR CHANGES IN INOCULATED CELL CULTURES WITH PESTIVIRUS, USING DIRECT IMMUNOFLURESCENCE TECHNIQUE

Author(s)

HEMATZADEH F. | KEYVANFAR H. | BADAVAM M. | TAHERI MOHAMMAD | Issue Writer Certificate

Keywords

PESTIVIRUSQ4

IMMUNOFLURESCENCEQ4

CYTOPATHIC STRAINQ4

NONCYTOPATHIC STRAINQ4

CELL CULTUREQ2

Abstract

Objective: This study was carried out to find the progressive cellular changes developed in CELL CULTURE with cytopathic (cp) and noncytopathic (ncp) PESTIVIRUSes isolated from cow and sheep. Design: Observational study. Procedure: Following inoculating the R-BK CELL CULTUREs in Lighton tube with NADL strain of BVD, a cp strain of Border disease virus and a nocytopathic BVD, the cells were stained with FA and observed with IF microscope. In inoculated cultures with cp and ncp PESTIVIRUS obvious changes were present at the first day and multiple focal expression of viral antigens were seen. Results: In the second day in cp inoculated cultures, the morphological changes began and antigenic spread were more intensive. In the 3rd day the CPE foci were seen in different parts of the cultured cells with high concentration of antigen around the CPE sites.-The cells started to detach and became rounds and finally in the 4th and 5th day, almost all cells were antigen positive and developed CPE. However, those cultures inoculated with NONCYTOPATHIC STRAIN of BVD, in daily observation the antigenic spread was too slow and up to 6 days post inoculation no CPE were observed.

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APA: Copy

HEMATZADEH, F., KEYVANFAR, H., BADAVAM, M., & TAHERI, MOHAMMAD. (2004). A STUDY ON CELLULAR CHANGES IN INOCULATED CELL CULTURES WITH PESTIVIRUS, USING DIRECT IMMUNOFLURESCENCE TECHNIQUE. JOURNAL OF VETERINARY RESEARCH, 59(2), 176-178. SID. https://sid.ir/paper/35137/en

Vancouver: Copy

HEMATZADEH F., KEYVANFAR H., BADAVAM M., TAHERI MOHAMMAD. A STUDY ON CELLULAR CHANGES IN INOCULATED CELL CULTURES WITH PESTIVIRUS, USING DIRECT IMMUNOFLURESCENCE TECHNIQUE. JOURNAL OF VETERINARY RESEARCH[Internet]. 2004;59(2):176-178. Available from: https://sid.ir/paper/35137/en

IEEE: Copy

F. HEMATZADEH, H. KEYVANFAR, M. BADAVAM, and MOHAMMAD TAHERI, “A STUDY ON CELLULAR CHANGES IN INOCULATED CELL CULTURES WITH PESTIVIRUS, USING DIRECT IMMUNOFLURESCENCE TECHNIQUE,” JOURNAL OF VETERINARY RESEARCH, vol. 59, no. 2, pp. 176–178, 2004, [Online]. Available: https://sid.ir/paper/35137/en

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