Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases

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SHOMALI AIDA; Mashayekhi Kambiz; Pahlevani Mohammad Hadi; MOUSAVIZADEH SEYYED JAVAD

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Journal: JOURNAL OF PLANT PHYSIOLOGY AND BREEDING Year:2018 | Volume:8 | Issue:2 Page(s): 53-58

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Information Journal Paper

Title

Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases

Author(s)

SHOMALI AIDA | Mashayekhi Kambiz | Pahlevani Mohammad Hadi | MOUSAVIZADEH SEYYED JAVAD | Issue Writer Certificate

Keywords

AmmoniumQ2

Casein hydrolysateQ1

In vitroQ1

NitrateQ2

TomatoQ2

Abstract

Tissue culture through somatic embryogenesis is one of the methods found most useful in the plants’ breeding process. A key issue to deal with during somatic evolution is its synchronization. Nitrogen has been known to play an important role here. Therefore, we evaluated the Tomato explant embryogenesis cultured on B5 basal medium, subject to oxidized, reduced and organic nitrogen. Two separate experiments were conducted consisting of eight treatments with four replications each, using completely randomized design. In the experiment in which treatments were applied during the induction phase, maximum synchrony, based on relative number of torpedo embryo to all formed embryos, was obtained using Nitrate as the sole nitrogen source (0. 81). However, in the case in which treatments were applied during the realization phase, maximum synchrony was obtained through the combined Nitrate and Casein hydrolysate (0. 51). Furthermore, in both experiments the highest number of somatic embryos was obtained in the standard B5 medium (91. 58 in the first experiment and 59. 19 in the second experiment).

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APA: Copy

SHOMALI, AIDA, Mashayekhi, Kambiz, Pahlevani, Mohammad Hadi, & MOUSAVIZADEH, SEYYED JAVAD. (2018). Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases. JOURNAL OF PLANT PHYSIOLOGY AND BREEDING, 8(2 ), 53-58. SID. https://sid.ir/paper/357119/en

Vancouver: Copy

SHOMALI AIDA, Mashayekhi Kambiz, Pahlevani Mohammad Hadi, MOUSAVIZADEH SEYYED JAVAD. Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases. JOURNAL OF PLANT PHYSIOLOGY AND BREEDING[Internet]. 2018;8(2 ):53-58. Available from: https://sid.ir/paper/357119/en

IEEE: Copy

AIDA SHOMALI, Kambiz Mashayekhi, Mohammad Hadi Pahlevani, and SEYYED JAVAD MOUSAVIZADEH, “Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases,” JOURNAL OF PLANT PHYSIOLOGY AND BREEDING, vol. 8, no. 2 , pp. 53–58, 2018, [Online]. Available: https://sid.ir/paper/357119/en

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