The In-vitro Cytotoxicity Effect of Aflatoxins B1 and G1 on Mice T lymphocyte

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KEIKHA N.; SOHRABI N.; TAGHIZADEH M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Veterinary Research & Biological Products Year:2020 | Volume:33 | Issue:3 (128) Page(s): 140-148

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Title

The In-vitro Cytotoxicity Effect of Aflatoxins B1 and G1 on Mice T lymphocyte

Author(s)

KEIKHA N. | SOHRABI N. | TAGHIZADEH M. | Issue Writer Certificate

Keywords

AspergillusQ2

AflatoxinQ2

CytotoxicityQ1

Cattle feedQ1

T lymphocyteQ1

Abstract

Aflatoxins are chemical toxic metabolites which are produced by Aspergillus species. This study was designed to investigate the Aflatoxines Cytotoxicity effects on mice T lymphocyte. In this work, four different standard and feed derived strains of A. parasiticus and A. flavus were selected. The levels of G1 and B1 Aflatoxins were determined by TLC and HPLC methods. For evaluation of cytotoxity effect, the different concentration of Aflatoxins were mixed by mice T cell line (EL4) on different time and the percentage of cell death were been evaluated by MTT assay and flowcytometry (FCM) method. Among different strains, the standard A. parasiticus and feed derived A. flavus have highest levels of G1 and B1. The most cytotoxity effect were seen on 1/10 concentration of A. parasiticus extracted Aflatoxins. These results shown feed derived Aspergillus could produce G1 and B1 Aflatoxins which have considerable Cytotoxicity effect on mice T lymphocytes.

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APA: Copy

KEIKHA, N., SOHRABI, N., & TAGHIZADEH, M.. (2020). The In-vitro Cytotoxicity Effect of Aflatoxins B1 and G1 on Mice T lymphocyte. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 33(3 (128) ), 140-148. SID. https://sid.ir/paper/367809/en

Vancouver: Copy

KEIKHA N., SOHRABI N., TAGHIZADEH M.. The In-vitro Cytotoxicity Effect of Aflatoxins B1 and G1 on Mice T lymphocyte. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2020;33(3 (128) ):140-148. Available from: https://sid.ir/paper/367809/en

IEEE: Copy

N. KEIKHA, N. SOHRABI, and M. TAGHIZADEH, “The In-vitro Cytotoxicity Effect of Aflatoxins B1 and G1 on Mice T lymphocyte,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 33, no. 3 (128) , pp. 140–148, 2020, [Online]. Available: https://sid.ir/paper/367809/en

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