The efficiency of the partial genome of Turnip mosaic virus (TuMV) on resistance induction in canola

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JAFARI MAJID; MOIENI AHMAD; SHAMS BAKHSH MASOUD

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Journal: Iranian Journal of Plant Protection Science Year:2019 | Volume:49 | Issue:2 Page(s): 165-175

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Title

The efficiency of the partial genome of Turnip mosaic virus (TuMV) on resistance induction in canola

Author(s)

JAFARI MAJID | MOIENI AHMAD | SHAMS BAKHSH MASOUD | Issue Writer Certificate

Keywords

PotyvirusQ1

recoveryQ2

short sequenceQ1

scoring systemQ1

Abstract

Turnip mosaic virus (TuMV) a species of the genus Potyvirus in the family Potyviridae, is one of the most prevalent viruses of canola fields in Iran. So far, commercial resistant lines or varieties against TuMV have not been known in Iran. In this research, the possibility of inducing resistant to TuMV in transgenic canola lines carrying a short fragment of the coat protein gene of the virus was evaluated. Therefore, a 130 nucleotide fragment of TuMV coat protein gene, as sense or antisense orientations, were cloned in the pFGC5941 vector independently and then were transformed to canola cotyledonary (Hayola R-Line 401 variety) explants using Agrobacterium tumefaciens strain LBA4404. Selection and confirmation of transgenic T0 and T1 lines were carried out via PCR and glufosinate ammonium herbicide (selective marker) resistance assay under greenhouse conditions. After virus inoculation to T1 lines, resistance assay was done by the scoring system. The obtained results confirmed that transgenic lines with sense construction did not show any resistant. However, several transgenic lines with antisense construction showed a different level of resistant to TuMV including delay in symptoms appearance and a decrease in symptoms severity (0-30% of T1 progeny) or symptoms recovery (10-66% of T1 progeny). This is the first report of increased resistant against TuMV via transformation of a short viral fragment sequence with the antisense orientation in canola.

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APA: Copy

JAFARI, MAJID, MOIENI, AHMAD, & SHAMS BAKHSH, MASOUD. (2019). The efficiency of the partial genome of Turnip mosaic virus (TuMV) on resistance induction in canola. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(2 ), 165-175. SID. https://sid.ir/paper/370951/en

Vancouver: Copy

JAFARI MAJID, MOIENI AHMAD, SHAMS BAKHSH MASOUD. The efficiency of the partial genome of Turnip mosaic virus (TuMV) on resistance induction in canola. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2019;49(2 ):165-175. Available from: https://sid.ir/paper/370951/en

IEEE: Copy

MAJID JAFARI, AHMAD MOIENI, and MASOUD SHAMS BAKHSH, “The efficiency of the partial genome of Turnip mosaic virus (TuMV) on resistance induction in canola,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 2 , pp. 165–175, 2019, [Online]. Available: https://sid.ir/paper/370951/en

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