Evaluation of genetic variations in saffron with more than three stigmas using SSR and ISSR molecular markers

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BEHDANI MOHAMMAD ALI; Izanloo Ali

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Saffron Agronomy and Technology Year:2019 | Volume:7 | Issue:3 Page(s): 347-357

Download Full-Text

Persian Verion

View:

296

Download:

Cites:

Information Journal Paper

Title

Evaluation of genetic variations in saffron with more than three stigmas using SSR and ISSR molecular markers

Author(s)

BEHDANI MOHAMMAD ALI | Izanloo Ali | Issue Writer Certificate

Keywords

EpigeneticsQ1

Genetic diversityQ2

PolymorphismQ2

Abstract

Saffron is the most valuable spice in the world. It is genetically a monomorphic clone. However, differences in phenotype and quality have been reported. The most important agro-economically phenotypic variation is the appearance of flowers with more than three stigmas. The main objective of this study was to study the genetic variability of saffron clones with more than 3 stigmas using SSR and ISSR molecular markers. In this research, saffron clones with more than three stigmas were collected along with the corm and the root from Saffron fields of Qaen and Sarayan, South Khorassan province, then transferred as a whole to the Biotechnology Laboratory of the Faculty of Agriculture, the University of Birjand. The number of stigmas in each flower was counted. Genomic DNA was extracted according to the CTAB method from leaves of the flower with more than three stigmas. Flower with more than three stigmas was larger and had more petals than ordinary ones. The most frequent number of flowers with more than three stigmas was related to four and five stigmas with 38%. Six-spike samples with a frequency of 14% were observed. Among the collected samples, only one specimen with seven stigmas was observed. Of the 48 tested ISSR primers on the bulk of DNA, only 16 primers had amplified bands and selected. The results of agarose gel electrophoresis for ISSR primers amplified the bands ranged from 100 to 1000 bp. By examining the bands formed for ISSR primers, no significant Polymorphism was observed between different clones of saffron. Therefore, based on this marker system, no sign of Genetic diversity was observed between clones with different number of stigmas. Among the tested SSR markers, 10 primer pairs showed amplified band among the clones. The results of correlation analysis based on Spearman correlation coefficient showed that there was no statistically significant correlation between microsatellite marker alleles and number of stigmas.

Cites

No record.

References

No record.

Cite

APA: Copy

BEHDANI, MOHAMMAD ALI, & Izanloo, Ali. (2019). Evaluation of genetic variations in saffron with more than three stigmas using SSR and ISSR molecular markers. SAFFRON AGRONOMY AND TECHNOLOGY, 7(3 ), 347-357. SID. https://sid.ir/paper/371576/en

Vancouver: Copy

BEHDANI MOHAMMAD ALI, Izanloo Ali. Evaluation of genetic variations in saffron with more than three stigmas using SSR and ISSR molecular markers. SAFFRON AGRONOMY AND TECHNOLOGY[Internet]. 2019;7(3 ):347-357. Available from: https://sid.ir/paper/371576/en

IEEE: Copy

MOHAMMAD ALI BEHDANI, and Ali Izanloo, “Evaluation of genetic variations in saffron with more than three stigmas using SSR and ISSR molecular markers,” SAFFRON AGRONOMY AND TECHNOLOGY, vol. 7, no. 3 , pp. 347–357, 2019, [Online]. Available: https://sid.ir/paper/371576/en

Related Journal Papers