Study of polymorphism in the ATP1A1 gene and its association with conception rate in Holstein and Sarabi breeds

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GHAFFARI M.; NEJATI JAVAREMI A.; SADEGHI M.; MORADI SHAHRBABAK M.

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Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2020 | Volume:14 | Issue:4 Page(s): 367-371

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Title

Study of polymorphism in the ATP1A1 gene and its association with conception rate in Holstein and Sarabi breeds

Author(s)

GHAFFARI M. | NEJATI JAVAREMI A. | SADEGHI M. | MORADI SHAHRBABAK M. | Issue Writer Certificate

Keywords

Heat stressQ1

HaplotypesQ1

SNPQ1

PCR-SSCPQ1

Abstract

The objective of this study was the detection of polymorphism in ATP1A1 gene and its association with conception rates in Holstein and Sarabi cows by PCR-SSCP method. For this purpose, blood samples were taken from 124 cows. DNA was extracted from whole blood using modified salting-out method and polymerase chain reactions were performed for amplification of 330bp fragment of the bovine ATP1A1gene. The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method was used to scan for the mutations within the amplified regions. Subsequently, a numbers of samples per each band pattern were sequenced for identification of Single Nucleotide Polymorphism (SNP). The results of the sequencing and alignment using available sequences in NCBI two SNPs in the ATP1A1 gene that one of those was located in exon14 and another one in intron14 area and the mutation located in exon area was along with no changes in amino acid and obtained protein of this gene. For evaluating of association between this locations and conception rate trait, the band pattern (haplotype) were considered in the model. The results showed that the observed Haplotypes in the ATP1A1 gene is not significantly correlated with conception rate.

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APA: Copy

GHAFFARI, M., NEJATI JAVAREMI, A., SADEGHI, M., & MORADI SHAHRBABAK, M.. (2020). Study of polymorphism in the ATP1A1 gene and its association with conception rate in Holstein and Sarabi breeds. MODERN GENETICS JOURNAL (MGJ), 14(4 ), 367-371. SID. https://sid.ir/paper/376718/en

Vancouver: Copy

GHAFFARI M., NEJATI JAVAREMI A., SADEGHI M., MORADI SHAHRBABAK M.. Study of polymorphism in the ATP1A1 gene and its association with conception rate in Holstein and Sarabi breeds. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2020;14(4 ):367-371. Available from: https://sid.ir/paper/376718/en

IEEE: Copy

M. GHAFFARI, A. NEJATI JAVAREMI, M. SADEGHI, and M. MORADI SHAHRBABAK, “Study of polymorphism in the ATP1A1 gene and its association with conception rate in Holstein and Sarabi breeds,” MODERN GENETICS JOURNAL (MGJ), vol. 14, no. 4 , pp. 367–371, 2020, [Online]. Available: https://sid.ir/paper/376718/en

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