The effect of methyl jasmonate on the relative expression of genes involved in the pathway of glycyrrhizin biosynthesis in cell suspension culture of licorice (Glycirrhiza glabra)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Allahdou M.; OMIDI M.; BIHAMTA M.; ABBASI A.R.; FAKHERI B.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2019 | Volume:14 | Issue:3 Page(s): 178-186

Download Full-Text

Persian Verion

View:

430

Download:

Cites:

Information Journal Paper

Title

The effect of methyl jasmonate on the relative expression of genes involved in the pathway of glycyrrhizin biosynthesis in cell suspension culture of licorice (Glycirrhiza glabra)

Author(s)

Keywords

licoriceQ2

gene expressionQ1

cell suspensionQ1

methyl jasmonateQ1

Abstract

The roots and stolons of Glycyrrhiza species constitute one of the most important crude drugs in the world and contain a large amount of glycyrrhizin, an oleanane-type triterpenoid saponin. In this study, relative expression of the genes involved in the biosynthesis pathway of this secondary metabolite, including bAS, SQS, CYP88D6 and CYP72A154, was investigated in the presence of methyl jasmonate in a culture of cell suspension of licorice. methyl jasmonate was added at a concentration of 100 μ M in a stationary growth phase to cell suspension. The control experiment had no methyl jasmonate. The cells were harvested at intervals 24, 48 and 72 hours’ . The experimental was done as split plot in time based on completely randomized design with two replications. The results showed that the two bAS and CYP88D6 genes had the highest expression levels in 48 h after the treatment. SQS and CYP72A154 genes, had the highest expression levels in 72 hours after treatment, but the expression of CYP72A154 didn’ t show significant difference comparing to control. The bAS and SQS genes showed higher expression than CYP88D6 and CYP72A154 genes, Because the bAS and SQS genes are involved in the initial pathway of biosynthesis and also are involved in the biosynthesis of other saponins, such as soyasaponin, while The CYP88D6 and CYP72A154 genes are involved in the final pathway biosynthesis for glycyrrhizin. The previous studies had been confirmed presence of glycyrrhizin in the root. In this study, the increased expression of the genes involved in the biosynthesis pathway of glycyrrhizin in cell suspension culture confirmed the presence of glycyrrhizin in cell cultures of licorice.

Cites

No record.

References

No record.

Cite

APA: Copy

Allahdou, M., OMIDI, M., BIHAMTA, M., ABBASI, A.R., & FAKHERI, B.A.. (2019). The effect of methyl jasmonate on the relative expression of genes involved in the pathway of glycyrrhizin biosynthesis in cell suspension culture of licorice (Glycirrhiza glabra). MODERN GENETICS JOURNAL (MGJ), 14(3 ), 178-186. SID. https://sid.ir/paper/378938/en

Vancouver: Copy

Allahdou M., OMIDI M., BIHAMTA M., ABBASI A.R., FAKHERI B.A.. The effect of methyl jasmonate on the relative expression of genes involved in the pathway of glycyrrhizin biosynthesis in cell suspension culture of licorice (Glycirrhiza glabra). MODERN GENETICS JOURNAL (MGJ)[Internet]. 2019;14(3 ):178-186. Available from: https://sid.ir/paper/378938/en

IEEE: Copy

M. Allahdou, M. OMIDI, M. BIHAMTA, A.R. ABBASI, and B.A. FAKHERI, “The effect of methyl jasmonate on the relative expression of genes involved in the pathway of glycyrrhizin biosynthesis in cell suspension culture of licorice (Glycirrhiza glabra),” MODERN GENETICS JOURNAL (MGJ), vol. 14, no. 3 , pp. 178–186, 2019, [Online]. Available: https://sid.ir/paper/378938/en

Related Journal Papers