Effect of RNA silencing suppressors of Barley yellow striate mosaic virus on expression levels of autophagy-related genes in Nicotiana benthamiana16c

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RABIEE S.; Afsharifar A.; IZADPANAH K.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2020 | Volume:55 | Issue:4 Page(s): 287-303

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Title

Effect of RNA silencing suppressors of Barley yellow striate mosaic virus on expression levels of autophagy-related genes in Nicotiana benthamiana16c

Author(s)

RABIEE S. | Afsharifar A. | IZADPANAH K. | Issue Writer Certificate

Keywords

AutophagyQ1

PhosphoproteinQ1

RNA silencing suppressorQ1

Abstract

Autophagy is a degradation process in eukaryotes through which damaged or unwanted intracellular components are degraded. This process is also involved in plant disease resistance, although its mechanisms are not precisely known. Autophagy is regulated by multiple Autophagy-related proteins (ATGs). In this study, we investigated the possible impact of two Barley yellow striate mosaic virus (BYSMV) proteins, i. e., Phosphoprotein (P) and ancillary protein 3 (P3), on four important genes involved in Autophagy (ATG2, ATG6, ATG7, and AGO1) in N. benthamiana. P and P3 genes were cloned in pCAMBIA-1302 vector under the control of the 2 × 35S promoter and Hemagglutinin tag. Constructs of each gene were agroinfiltrated in the abaxial side of the N. benthamiana leaves. Five days after agroinfiltration, the expression level of these genes was measured using RT-qPCR. The results showed that expression of ATG2, ATG6 and ATG7 genes increased in all treatments (P, P3, P+P3). The level of ATG2 expression was 5. 57, 15. 6 and 5. 6 fold, in P/GFP, P3/GFP and P/P3/GFP treatments, respectively, while a 1. 5-fold reduction was obtained in expression of AGO1 in all treatments. These results implied that P and P3 proteins of BYSMV can modify the expression of Autophagy related genes in N. benthamiana plant. These findings suggest the involvement of AGO1, ATG6, ATG7 and ATG2 in immune responses of N. benthamiana against BYSMV, which provide a better understanding of plant host defense mechanisms against virus infections and might be an opportunity to exploit a novel antivirus approach.

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APA: Copy

RABIEE, S., Afsharifar, A., & IZADPANAH, K.. (2020). Effect of RNA silencing suppressors of Barley yellow striate mosaic virus on expression levels of autophagy-related genes in Nicotiana benthamiana16c. IRANIAN JOURNAL OF PLANT PATHOLOGY, 55(4 ), 287-303. SID. https://sid.ir/paper/379192/en

Vancouver: Copy

RABIEE S., Afsharifar A., IZADPANAH K.. Effect of RNA silencing suppressors of Barley yellow striate mosaic virus on expression levels of autophagy-related genes in Nicotiana benthamiana16c. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2020;55(4 ):287-303. Available from: https://sid.ir/paper/379192/en

IEEE: Copy

S. RABIEE, A. Afsharifar, and K. IZADPANAH, “Effect of RNA silencing suppressors of Barley yellow striate mosaic virus on expression levels of autophagy-related genes in Nicotiana benthamiana16c,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 55, no. 4 , pp. 287–303, 2020, [Online]. Available: https://sid.ir/paper/379192/en

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