Identification and molecular cloning of defensin antimicrobial genes from six lentil genotypes

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Mir Derikvand Reza; SOHRABI SEYYED MOHSEN; SAMIEI KAMRAN

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Journal: Crop Biotechnology Year:2018 | Volume:7 | Issue:21 Page(s): 59-70

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Title

Identification and molecular cloning of defensin antimicrobial genes from six lentil genotypes

Author(s)

Mir Derikvand Reza | SOHRABI SEYYED MOHSEN | SAMIEI KAMRAN | Issue Writer Certificate

Keywords

Antimicrobial peptidesQ1

defensinsQ2

gene isolationQ1

intronQ2

lentilQ2

Abstract

Plant defensins are a large family of cysteine-rich Antimicrobial peptides. These peptides are small molecules with molecular mass between 5 and 7kD and eight conserved cysteines that are involved in disulfide bond formation. In the present study, some members of defensin gene family were isolated and characterized from lentil genotypes. All coding sequences of the defensin genes of dicotyledon plants were retrieved from GenBank. The retrieved coding sequences were aligned against lentil EST library and the resulted sequences were pooled and assembled. The resulted contigs and singletons were aligned against GenBank nr database using BLASTn tool. The contigs and singletons with the full open reading frame were used to primer design. In the next step, DNA extraction was performed from Gachsaran, Mahali, Filip2003-2L, Filip2003-9L, Filip2005-2L and Filip2006-10L genotypes. Finally, by using PCR, genomic sequence of defensins were amplified, cloned into pTZ57R/T plasmid and sequenced. The results showed complete identity among the isolated defensins from all genotypes. The identified genes also contain conserved introns with variable length that comprised of regulatory elements for response to different factors and conditions. For first time in this study, genomic sequences of three members of defensin gene family were isolated from lentil genotypes and structures and features of them were determined.

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APA: Copy

Mir Derikvand, Reza, SOHRABI, SEYYED MOHSEN, & SAMIEI, KAMRAN. (2018). Identification and molecular cloning of defensin antimicrobial genes from six lentil genotypes. JOURNAL OF CROP BIOTECHNOLOGY, 7(21 ), 59-70. SID. https://sid.ir/paper/380812/en

Vancouver: Copy

Mir Derikvand Reza, SOHRABI SEYYED MOHSEN, SAMIEI KAMRAN. Identification and molecular cloning of defensin antimicrobial genes from six lentil genotypes. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2018;7(21 ):59-70. Available from: https://sid.ir/paper/380812/en

IEEE: Copy

Reza Mir Derikvand, SEYYED MOHSEN SOHRABI, and KAMRAN SAMIEI, “Identification and molecular cloning of defensin antimicrobial genes from six lentil genotypes,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 7, no. 21 , pp. 59–70, 2018, [Online]. Available: https://sid.ir/paper/380812/en

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