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Information Journal Paper

Title

Subcellular localization of silencing suppressor proteins encoded by two cereal yellow dwarf viruses

Author(s)

 Almasi R. | Issue Writer Certificate 

Pages

  227-235

Abstract

 Cereal yellow dwarf viruses are among the most destructive cereal viruses worldwide. In this study, subcellular localizations of silencing suppressor proteins ( P0) from two cereal yellow dwarf viruses, Cereal yellow dwarf virus-RPV (CYDV-RPV) and Cereal yellow dwarf virus-RPS (CYDV-RPS), were examined via confocal microscopy. To do so, P0 genes were synthesized by polymerase chain reaction (PCR) and were cloned through Gateway cloning system. Agrobacterium. tumefaciens clones harboring GFP: P0 constructs were infiltrated into Nicotiana benthamiana plants. Transient expression of P0 proteins with N-terminal GPF fusion enabled us to examine their subcellular localizations in plant cells, 24 hours post infiltration. Results showed that both proteins are nuclear-cytoplasmic but different tendencies were observed in their localizations. In other words, P0 of CYDV-RPS was more nuclear oriented than P0 of CYDV-RPV. In the Western blot analysis using GFP polyclonal antibody only GFP: P0 proteins but no free GFP, were detected indicating that localizations were related to GFP: P0 proteins.

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    • Cite

    APA: Copy

    Almasi, R.. (2019). Subcellular localization of silencing suppressor proteins encoded by two cereal yellow dwarf viruses. IRANIAN JOURNAL OF PLANT PATHOLOGY, 55(3 ), 227-235. SID. https://sid.ir/paper/381744/en

    Vancouver: Copy

    Almasi R.. Subcellular localization of silencing suppressor proteins encoded by two cereal yellow dwarf viruses. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2019;55(3 ):227-235. Available from: https://sid.ir/paper/381744/en

    IEEE: Copy

    R. Almasi, “Subcellular localization of silencing suppressor proteins encoded by two cereal yellow dwarf viruses,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 55, no. 3 , pp. 227–235, 2019, [Online]. Available: https://sid.ir/paper/381744/en

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