Study of proteome enzymes involved in mycoparasitism process of Trichoderma harizanum mutant isolate

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NASERIPOUR TABASOM; Nasrollahnejad Saeed; SHAHBAZI SAMIRA; RAHNAMA KAMRAN

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Journal: BIO CONTROL IN PLANT PROTECTION Year:2019 | Volume:6 | Issue:2 (12) Page(s): 15-30

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Title

Study of proteome enzymes involved in mycoparasitism process of Trichoderma harizanum mutant isolate

Author(s)

NASERIPOUR TABASOM | Nasrollahnejad Saeed | SHAHBAZI SAMIRA | RAHNAMA KAMRAN | Issue Writer Certificate

Keywords

TrichodermaQ1

gamma-rayQ1

SDS-PAGEQ1

2D electrophoresisQ1

chitinaseQ1

Abstract

This study was conducted to investigate the expression pattern of chitinase in mutant strains of the fungus Trichoderma harizanum, to the wildisolate. For this purpose, the strains of T. harizanum were subjected to mutation with optimum doses of 250 Gy of gamma radiation. Both the anti-mutant and wild strains were studiedwith the dual culture on the plant pathogen Sclerotinia sclerotiorum. All statistical analyzes were performed with SAS software and test least significant difference (LSD). The profile protein of produced enzymes by all strains using SDS-PAGE electrophoresis and proteome pattern of wildisolate with Th M8 mutant strains by colloidal chitin substrate was investigated using two-dimensional electrophoresis techniques and software ImageMaster 2D Platinum. The results showed that the majority of mutant strains compared to wild strains in the production of extracellular enzyme chitinase, were significantly different in the 1% and 5%. The results of the test antagonist showed that the potential control mutant strains compared to wild strains of the fungus S. sclerotiorum cope with significantly increased. The highest and lowest inhibition is related to strain ThM8 to 72% and ThM3 to 43%, respectively. On the other hand, the proteome pattern based on, twodimensional electrophoresis technique showed that the protein spots of mutant strains compared to wild type isolates different both quantitatively and qualitatively. This suggests that mutations can lead to a change in the pattern of expression of the protein is secreted. The results of this study showed that gamma irradiation techniques can be effective in the production of enzymes in maycoparasitism and improvement biological control potential of plant diseases to be effective in management.

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APA: Copy

NASERIPOUR, TABASOM, Nasrollahnejad, Saeed, SHAHBAZI, SAMIRA, & RAHNAMA, KAMRAN. (2019). Study of proteome enzymes involved in mycoparasitism process of Trichoderma harizanum mutant isolate. BIO CONTROL IN PLANT PROTECTION, 6(2 (12) ), 15-30. SID. https://sid.ir/paper/388700/en

Vancouver: Copy

NASERIPOUR TABASOM, Nasrollahnejad Saeed, SHAHBAZI SAMIRA, RAHNAMA KAMRAN. Study of proteome enzymes involved in mycoparasitism process of Trichoderma harizanum mutant isolate. BIO CONTROL IN PLANT PROTECTION[Internet]. 2019;6(2 (12) ):15-30. Available from: https://sid.ir/paper/388700/en

IEEE: Copy

TABASOM NASERIPOUR, Saeed Nasrollahnejad, SAMIRA SHAHBAZI, and KAMRAN RAHNAMA, “Study of proteome enzymes involved in mycoparasitism process of Trichoderma harizanum mutant isolate,” BIO CONTROL IN PLANT PROTECTION, vol. 6, no. 2 (12) , pp. 15–30, 2019, [Online]. Available: https://sid.ir/paper/388700/en

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