Improvement of Cellulose Degrading Enzymes Activity by Mutagenesis in Trichoderma reesei Fungi

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ASKARI HAMED; SHAHBAZI SAMIRA

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Journal: Agricultural Biotechnology Year:2019 | Volume:17 | Issue:2 Page(s): 41-50

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Information Journal Paper

Title

Improvement of Cellulose Degrading Enzymes Activity by Mutagenesis in Trichoderma reesei Fungi

Author(s)

ASKARI HAMED | SHAHBAZI SAMIRA | Issue Writer Certificate

Keywords

TrichodermaQ1

Cellulase enzymeQ1

SDS-PAGEQ1

Gamma radiationQ1

MutationQ2

Abstract

Trichoderma reesei is one of the most important fungi producing cellulose degrading enzymes in nature. In this study, 21 mutated isolate via gamma irradiation of T. reesei were screened for Cellulase enzyme production on a colloidal cellulose medium. The concentration of extracellular proteins of wild and mutant isolates were measured using Bradford's method. Avicel, carboxymethyl cellulose and filter paper were used to measure cellulase activity. The purity and composition of enzyme-rich proteins were also evaluated using polyacrylamide gel electrophoresis (SDS-PAGE) test. The highest amount of extracellular protein production was observed in T. r M7 and T. r M17 isolates. Activity of endoglucanase, exoglucanase and total Cellulase enzymes in mutated isolate T. r M8 showed the highest levels of enzymatic activity among mutant and wild isolates. The difference in the molecular weight of the enzyme bands showed that the EG 4, Cel 1A, Cel 12A, Cel 45A, Cel 3A, Cel 7A, Cel 6A, Cel 5A and Cel 61A enzymes hydrolyzed the colloidal cellulose, synergictly. These results indicate that, Mutation induced by Gamma radiation is possible to obtain high producing Trichoderma mutants of Cellulase enzymes.

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APA: Copy

ASKARI, HAMED, & SHAHBAZI, SAMIRA. (2019). Improvement of Cellulose Degrading Enzymes Activity by Mutagenesis in Trichoderma reesei Fungi. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), 17(2 ), 41-50. SID. https://sid.ir/paper/368944/en

Vancouver: Copy

ASKARI HAMED, SHAHBAZI SAMIRA. Improvement of Cellulose Degrading Enzymes Activity by Mutagenesis in Trichoderma reesei Fungi. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH)[Internet]. 2019;17(2 ):41-50. Available from: https://sid.ir/paper/368944/en

IEEE: Copy

HAMED ASKARI, and SAMIRA SHAHBAZI, “Improvement of Cellulose Degrading Enzymes Activity by Mutagenesis in Trichoderma reesei Fungi,” AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), vol. 17, no. 2 , pp. 41–50, 2019, [Online]. Available: https://sid.ir/paper/368944/en

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