Designing Dot ELISA kit for detection of Staphylococcus aureus SEA enterotoxin and Sandwich ELISA compare, its validation in pastry cream bread

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Bathaei Sara Alsadat; AHARI HAMED; Anvar Seyed Amir Ali

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Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2020 | Volume:17 | Issue:2 (69) Page(s): 3193-3202

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Title

Designing Dot ELISA kit for detection of Staphylococcus aureus SEA enterotoxin and Sandwich ELISA compare, its validation in pastry cream bread

Author(s)

Bathaei Sara Alsadat | AHARI HAMED | Anvar Seyed Amir Ali | Issue Writer Certificate

Keywords

EnterotoxinQ1

Staphylococcus aureusQ1

Dot ELISAQ1

KitQ1

Abstract

In this study, detection and recognition of Staphylococcus aureus Enterotoxin was performed by designing a Dot ELISA sensitive Kit. In the present study, nitrocellulose membrane (NCM) with 0. 22 micron pores was applied as the bed, and 1: 500 diluted specific antibody of Staphylococcus aureus Enterotoxin was coated on the NCM. Next, The HRP-tagged antibody was added with dilution of 1/2000, and the substrate was added gradually and rewashed. In this experiment, the samples were manually contaminated and then compared with the ELISA sandwich method as a gold standard. The Kit is capable of detecting 50 ng/ml and higher of toxin A, and if the amount of toxin is less, it will report it negatively. In this test, of which 40 samples were collected and 4 positive cases were reported, it was revealed that about 10% of the collected samples were contaminated. Nowadays, using the Dot ELISA Kit as a quick method with relative sensitivity and properties, it is possible to improve the repeatability of detecting Staphylococcus Enterotoxin A in creamy candies.

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APA: Copy

Bathaei, Sara Alsadat, AHARI, HAMED, & Anvar, Seyed Amir Ali. (2020). Designing Dot ELISA kit for detection of Staphylococcus aureus SEA enterotoxin and Sandwich ELISA compare, its validation in pastry cream bread. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 17(2 (69) ), 3193-3202. SID. https://sid.ir/paper/390586/en

Vancouver: Copy

Bathaei Sara Alsadat, AHARI HAMED, Anvar Seyed Amir Ali. Designing Dot ELISA kit for detection of Staphylococcus aureus SEA enterotoxin and Sandwich ELISA compare, its validation in pastry cream bread. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2020;17(2 (69) ):3193-3202. Available from: https://sid.ir/paper/390586/en

IEEE: Copy

Sara Alsadat Bathaei, HAMED AHARI, and Seyed Amir Ali Anvar, “Designing Dot ELISA kit for detection of Staphylococcus aureus SEA enterotoxin and Sandwich ELISA compare, its validation in pastry cream bread,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 17, no. 2 (69) , pp. 3193–3202, 2020, [Online]. Available: https://sid.ir/paper/390586/en

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