Molecular Detection of Avian Metapneumovirus in Semnan Broiler Farms

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Darebaghi Amir; Emadi Chashmi Seyed Hesamodin; Kafshdozan Khatereh; HOSSEINI HOSSEIN

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Journal: Iranian Journal of Veterinary Medicine Year:2021 | Volume:15 | Issue:1 Page(s): 27-34

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Information Journal Paper

Title

Molecular Detection of Avian Metapneumovirus in Semnan Broiler Farms

Author(s)

Darebaghi Amir | Emadi Chashmi Seyed Hesamodin | Kafshdozan Khatereh | HOSSEINI HOSSEIN | Issue Writer Certificate

Keywords

Avian MetapneumovirusQ1

Broiler flocksQ1

Nd/Nx primerQ1

RT-PCRQ1

Subtype BQ1

Abstract

BACKGROUND: Avian Metapneumovirus (AMPV) causes mild to acute contagious infection of the upper respiratory tract in turkey and chicken with different mortality rate. OBJECTIVES: This study was aimed to molecular detection and subtyping of AMPV infection in Broiler flocks using RT-PCR method in Semnan province on samples from 2016 to2020. METHODS: Sampling was carried out from the upper part of the trachea, choana, and sinuses of broiler chickens from the 85 Broiler flocks. All flocks were more than 3 weeks of age. In total 10 swabs were taken from each flock, while each 5 were pooled as one sample (total two samples per flock). The samples were transferred to the laboratory for RNA extraction and RT-PCR amplification. RESULTS: Out of 85 tested Broiler flocks, 30 (35. 3%) were positive for AMPV using the Nd/Nx primer set. In addition, 28 positive samples were found to be of Subtype B using the Ga/G12 primer set and 2 remaining positive samples were non-Subtype B, probably A, C or D subtypes. CONCLUSIONS: Since AMPV vaccination was not performed in Semnan province, it can be concluded that some cases were infected with the natural viruses. Therefore, vaccination could be effective in controlling AMPV-induced respiratory distress.

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APA: Copy

Darebaghi, Amir, Emadi Chashmi, Seyed Hesamodin, Kafshdozan, Khatereh, & HOSSEINI, HOSSEIN. (2021). Molecular Detection of Avian Metapneumovirus in Semnan Broiler Farms. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 15(1 ), 27-34. SID. https://sid.ir/paper/390748/en

Vancouver: Copy

Darebaghi Amir, Emadi Chashmi Seyed Hesamodin, Kafshdozan Khatereh, HOSSEINI HOSSEIN. Molecular Detection of Avian Metapneumovirus in Semnan Broiler Farms. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2021;15(1 ):27-34. Available from: https://sid.ir/paper/390748/en

IEEE: Copy

Amir Darebaghi, Seyed Hesamodin Emadi Chashmi, Khatereh Kafshdozan, and HOSSEIN HOSSEINI, “Molecular Detection of Avian Metapneumovirus in Semnan Broiler Farms,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 15, no. 1 , pp. 27–34, 2021, [Online]. Available: https://sid.ir/paper/390748/en

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