Investigating of the Influence of Cellulase Enzymes from Mutated Isolates of Trichoderma harzianum and T. viride on Biodegradation of Cellulose Iα , Iβ and III

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SHAHBAZI SAMIRA; ASKARI HAMED

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Journal: Agricultural Biotechnology Year:2018 | Volume:17 | Issue:1 Page(s): 35-44

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Title

Investigating of the Influence of Cellulase Enzymes from Mutated Isolates of Trichoderma harzianum and T. viride on Biodegradation of Cellulose Iα , Iβ and III

Author(s)

SHAHBAZI SAMIRA | ASKARI HAMED | Issue Writer Certificate

Keywords

CrystallinityQ1

AvicelQ1

Bactrial celluloseQ1

Cellulase enzymeQ1

MutantQ1

Abstract

In this research, two strain of Trichoderma (T. harzianum and T. viride) and their Mutants were used for Cellulase enzyme production. The Avicel, CMC(III), Bacterial Cellulose (Iα ) and Whatman NO. 1 filter paper, were used for cellulase activity assay. Their chemical structural properties were investigated by FT-IR, XRD and SEM compared to Avicel. The molecular weight of Cellulase enzymes were studied using SDS-PAGE. The SEM image of substrates, showed more delicacy of BC fibers relative to Avicel and CMC. The diameter ratio of BC to Avicel is approximately 1/30 or less. The FTIR spectroscopy and XRD assessments designated that the produced CMC is carrying a carboxylic group and its Crystallinity is 81. 84. The Crystallinity of the Avicel and BC were demonstrated that the Crystallinity index of Avicel (89. 15%) was more than that of bacterial cellulose (66. 44%). Both species and their Mutants produced varying amounts of extracellular proteins in the fermentation medium. In T. harzianum and its Mutants, the highest Cellulase enzyme activity were showed in Th M7 and Th M6, respectively and in T. viride and its Mutants, the highest enzymatic activity of Mutant strains was observed in Tv M14, Tv M15, respectively. The results showed that bacterial cellulose is a good substrate for total cellulase activity assay, because it has the highest enzyme activity compared to filter paper and Avicel; so it signs a high potential of accessibility for cellulose Iα biodegradability compared to cellulose Iβ .

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APA: Copy

SHAHBAZI, SAMIRA, & ASKARI, HAMED. (2018). Investigating of the Influence of Cellulase Enzymes from Mutated Isolates of Trichoderma harzianum and T. viride on Biodegradation of Cellulose Iα , Iβ and III. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), 17(1 ), 35-44. SID. https://sid.ir/paper/398058/en

Vancouver: Copy

SHAHBAZI SAMIRA, ASKARI HAMED. Investigating of the Influence of Cellulase Enzymes from Mutated Isolates of Trichoderma harzianum and T. viride on Biodegradation of Cellulose Iα , Iβ and III. AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH)[Internet]. 2018;17(1 ):35-44. Available from: https://sid.ir/paper/398058/en

IEEE: Copy

SAMIRA SHAHBAZI, and HAMED ASKARI, “Investigating of the Influence of Cellulase Enzymes from Mutated Isolates of Trichoderma harzianum and T. viride on Biodegradation of Cellulose Iα , Iβ and III,” AGRICULTURAL BIOTECHNOLOGY (AGRICULTURAL RESEARCH), vol. 17, no. 1 , pp. 35–44, 2018, [Online]. Available: https://sid.ir/paper/398058/en

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