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Information Journal Paper

Title

The antimicrobial effect of Stachysbyzantina extract on pathogenic bacteria using agar dilution and Cylinder-plate methods

Pages

  33-42

Abstract

 Stachysbyzantina belongs to Lamiaceae family. Regarding to the biologically active compounds and traditional use of the Stachysbyzantina for treatment of wounds and a cold, it seems that this plant has significant antibacterial effects. The aim of this study was evaluation of antibacterial effect of ethanolic extract of Stachysbyzantinaagainst Enterobacteraerogenes, Escherichia coli, Bacillus cereus and Staphylococcus aureus. The inhibition zone diameter of the Stachysbyzantina extract on Enterobacter aerogenes, Escherichia coli, Bacillus cereus and Staphylococcus aureus was identified on a concentration of 25, 50, and 75 mg/mL, respectively, by Cylinder-plate and Kirby-Bauer methods. Moreover, minimum inhibitory concentration (MIC) (Agar dilution and micro-well dilution) and minimum bactericidal concentration (MBC) of the pathogenic microorganisms were determined in this study. The data were analyzed using one-way ANOVA and Duncan's multiple range test. The highest and lowest inhibition zone with a diameter of 17. 90 and 13. 10 mm at a concentration of 75 mg/mL were observed for Staphylococcus aureus and Enterobacter aerogenes bacteria, respectively (Cylinder-plate method). The MIC of Stachysbyzantinaextract for Enterobacter aerogenes, Escherichia coli, Bacillus cereus and Staphylococcus aureus was 100, 50, 25 and 12. 5 mg/mL respectively (Agar dilution). The ethanolic extract of Stachysbyzantina“ in vitro” has a significant antibacterial effect on Enterobacter aerogenes and Escherichia coli (Gram-negative bacteria)and Bacillus cereus and Staphylococcus aureus (Gram-positive bacteria).

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    APA: Copy

    NOSHAD, M., & Alizadehbehbahani, B.. (2021). The antimicrobial effect of Stachysbyzantina extract on pathogenic bacteria using agar dilution and Cylinder-plate methods. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 17(109 ), 33-42. SID. https://sid.ir/paper/401439/en

    Vancouver: Copy

    NOSHAD M., Alizadehbehbahani B.. The antimicrobial effect of Stachysbyzantina extract on pathogenic bacteria using agar dilution and Cylinder-plate methods. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2021;17(109 ):33-42. Available from: https://sid.ir/paper/401439/en

    IEEE: Copy

    M. NOSHAD, and B. Alizadehbehbahani, “The antimicrobial effect of Stachysbyzantina extract on pathogenic bacteria using agar dilution and Cylinder-plate methods,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 17, no. 109 , pp. 33–42, 2021, [Online]. Available: https://sid.ir/paper/401439/en

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