THERMODYNAMIC STUDY OF CARBOXY TERMINAL OF BOTULINUM NEUROTOXIN TYPE E BY CIRCULAR DICHROISM SPECTROSCOPY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ROSTAMIAN M.; MOUSAVI S.J.; EBRAHIMI F.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Advanced Defense Science and Technology Year:2013 | Volume:4 | Issue:1 (11) Page(s): 31-38

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

778

Download:

Cites:

Information Journal Paper

Title

THERMODYNAMIC STUDY OF CARBOXY TERMINAL OF BOTULINUM NEUROTOXIN TYPE E BY CIRCULAR DICHROISM SPECTROSCOPY

Author(s)

ROSTAMIAN M. | MOUSAVI S.J. | EBRAHIMI F. | Issue Writer Certificate

Keywords

BOTULINUM NEUROTOXIN TYPE EQ3

RECOMBINANTQ3

Abstract

Nowadays for prevention of botulism syndrome, researchers have tended to use RECOMBINANT vaccines. One of these vaccines is a RECOMBINANT protein containing 93 amino acids of C-terminal of BOTULINUM NEUROTOXIN TYPE E (rBoNT/EHCC). In this study, after expression and purification of rBoNT/E-HCC, the effect of different pH on the protein was carried out by Circular dichroism (CD) spectrometry and followed by thermodynamics studies. The main goal was, to find the optimum purification condition for this protein and also to evaluate the possibility of using this protein as an oral vaccine. For this purpose, thermal danaturation of rBoNT/E-HCC was studied at four different pH (2, 5, 7.4, and 9) by CD. Our results indicated that Tm and DGo of rBoNT/E-HCC are higher in alkaline pH in comparison with acidic pH. Finally, our results suggest that; neutral or alkaline pH as the optimum pH for rBoNT/E-HCC purification.

Cites

No record.

References

No record.

Cite

APA: Copy

ROSTAMIAN, M., MOUSAVI, S.J., & EBRAHIMI, F.. (2013). THERMODYNAMIC STUDY OF CARBOXY TERMINAL OF BOTULINUM NEUROTOXIN TYPE E BY CIRCULAR DICHROISM SPECTROSCOPY. (JOURNAL OF ADVANCED DEFENCE SCIENCE AND TECHNOLOGY) JOURNAL OF PASSIVE DEFENCE SCIENCE AND TECHNOLOGY, 4(1 (11)), 31-38. SID. https://sid.ir/paper/405775/en

Vancouver: Copy

ROSTAMIAN M., MOUSAVI S.J., EBRAHIMI F.. THERMODYNAMIC STUDY OF CARBOXY TERMINAL OF BOTULINUM NEUROTOXIN TYPE E BY CIRCULAR DICHROISM SPECTROSCOPY. (JOURNAL OF ADVANCED DEFENCE SCIENCE AND TECHNOLOGY) JOURNAL OF PASSIVE DEFENCE SCIENCE AND TECHNOLOGY[Internet]. 2013;4(1 (11)):31-38. Available from: https://sid.ir/paper/405775/en

IEEE: Copy

M. ROSTAMIAN, S.J. MOUSAVI, and F. EBRAHIMI, “THERMODYNAMIC STUDY OF CARBOXY TERMINAL OF BOTULINUM NEUROTOXIN TYPE E BY CIRCULAR DICHROISM SPECTROSCOPY,” (JOURNAL OF ADVANCED DEFENCE SCIENCE AND TECHNOLOGY) JOURNAL OF PASSIVE DEFENCE SCIENCE AND TECHNOLOGY, vol. 4, no. 1 (11), pp. 31–38, 2013, [Online]. Available: https://sid.ir/paper/405775/en

Related Journal Papers