Optimization of rapid and efficient hairy root induction in two Hyoscyamus reticulatus L. and Hyoscyamus pusillus L.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHODSI R.; HOSSEINI B.; HEDAYATI A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS Year:2020 | Volume:36 | Issue:1 (99) Page(s): 22-39

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

355

Download:

Cites:

Information Journal Paper

Title

Optimization of rapid and efficient hairy root induction in two Hyoscyamus reticulatus L. and Hyoscyamus pusillus L.

Author(s)

GHODSI R. | HOSSEINI B. | HEDAYATI A. | Issue Writer Certificate

Keywords

Agrobacterium rhizogenesisQ1

basal media cultureQ1

total phenol and flavonoidQ1

hairy rootQ1

Abstract

Hyoscyamus species such as H. reticulatus L. and H. pusillus L. are rich sources of tropane alkaloids, mainly hyoscyamine and scopolamine, which are used for their mydriatic, antispasmodic, anticholinergic, analgesic and sedative properties. In this study, in the first experiment, the effects of four strains of Agrobacterium rhizogenes (ATCC 15834, A4, A7 and A13) and three explants (leaf, hypocotyls, and cotyledon) on hairy root induction were investigated in both H. reticulatus and H. pusillus species. In the second experiment, the effects of various culture media (MS, 1/2 MS, 1/4 MS, and B5) were evaluated on some morphological and phytochemical traits of hairy roots. The results showed that in H. reticulatus, maximum hairy root induction (78%) was obtained in the cotyledon explant inoculated with A7 strain and maximum hairy roots fresh weight (6. 25 g) and dry weight (0. 5 g) were recorded in MS medium. In this species, the highest total phenol (3. 82 mg GAE g-1 FW) and total flavonoid (7 mg QUE g-1 FW) content were observed in MS medium; however, no significant changes were observed in the activity of antioxidant enzymes of hairy roots cultured in different media. In H. pusillus, the leaf explant inoculated with A13 strain showed maximum hairy root induction (90%), and the highest hairy root fresh weight (5. 25 g) and dry weight (0. 43 g) were observed in MS medium and the highest total phenol (2. 26 mg GAE g-1 FW) and flavonoid (9. 22 mg QUE g-1 FW) content were obtained in B5 medium. This study showed that basal culture medium type and plant species had a significant impact on the biomass and phytochemical characteristics of hairy roots.

Cites

No record.

References

No record.

Cite

APA: Copy

GHODSI, R., HOSSEINI, B., & HEDAYATI, A.. (2020). Optimization of rapid and efficient hairy root induction in two Hyoscyamus reticulatus L. and Hyoscyamus pusillus L.. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, 36(1 (99) ), 22-39. SID. https://sid.ir/paper/407546/en

Vancouver: Copy

GHODSI R., HOSSEINI B., HEDAYATI A.. Optimization of rapid and efficient hairy root induction in two Hyoscyamus reticulatus L. and Hyoscyamus pusillus L.. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS[Internet]. 2020;36(1 (99) ):22-39. Available from: https://sid.ir/paper/407546/en

IEEE: Copy

R. GHODSI, B. HOSSEINI, and A. HEDAYATI, “Optimization of rapid and efficient hairy root induction in two Hyoscyamus reticulatus L. and Hyoscyamus pusillus L.,” IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, vol. 36, no. 1 (99) , pp. 22–39, 2020, [Online]. Available: https://sid.ir/paper/407546/en

Related Journal Papers