Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein gene by pET24-G1 recombinant construct in Escherichia coli

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PASANDIDEH R.; BEIGI NASSIRI M.T.; SEYFI ABAD SHAPOURI M.R.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2019 | Volume:15 | Issue:1 Page(s): 15-24

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Title

Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein gene by pET24-G1 recombinant construct in Escherichia coli

Author(s)

PASANDIDEH R. | BEIGI NASSIRI M.T. | SEYFI ABAD SHAPOURI M.R. | Issue Writer Certificate

Keywords

GeneQ1

Bovine Ephemeral Fever VirusQ1

Recombinant G1 proteinQ2

Escherichia coliQ2

Abstract

Bovine ephemeral fever (BEF) is a viral disease of cattle and water buffalo seen in some provinces of Iran, Generally southern and warm regions in recent years. The G glycoprotein is the main protective antigen of the Bovine Ephemeral Fever Virus (BEFV) and the target of anti-BEFV neutralizing antibodies. The aim of the present study was cloning and expression of the G1 epitope of BEF virus G glycoprotein Gene in a prokaryotic system. For this purpose, the G1 epitope was cloned in a prokaryotic expression vector, pET-24a(+), under the control of the T7 promoter and subsequently the recombinant pET24-G1 construct was transformed into Rosetta strain of Escherichia coli. Expressed recombinant protein was analyzed using SDS-PAGE and immunoblotting methods. SDS-PAGE analysis showed that a protein with ~18 kDa molecular weight, consistent with the expected molecular weight of recombinant protein fused to 6xHis tag, was expressed. Immunoblotting analysis showed that the expressed G1 protein specifically reacted with a mouse polyclonal serum against BEFV. Thus, in this study the G1 protein of BEFV was successfully expressed by the pET24-G1 recombinant construct in Escherichia coli. Based on the results of this study, immunization and the efficacy of this product can be consider as a possible candidate for the production of subunit vaccine against the virus in animal models.

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APA: Copy

PASANDIDEH, R., BEIGI NASSIRI, M.T., & SEYFI ABAD SHAPOURI, M.R.. (2019). Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein gene by pET24-G1 recombinant construct in Escherichia coli. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 15(1 ), 15-24. SID. https://sid.ir/paper/407820/en

Vancouver: Copy

PASANDIDEH R., BEIGI NASSIRI M.T., SEYFI ABAD SHAPOURI M.R.. Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein gene by pET24-G1 recombinant construct in Escherichia coli. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2019;15(1 ):15-24. Available from: https://sid.ir/paper/407820/en

IEEE: Copy

R. PASANDIDEH, M.T. BEIGI NASSIRI, and M.R. SEYFI ABAD SHAPOURI, “Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein gene by pET24-G1 recombinant construct in Escherichia coli,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 15, no. 1 , pp. 15–24, 2019, [Online]. Available: https://sid.ir/paper/407820/en

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