IDENTIFICATION OF WHITE SPOT SYNDROME DISEASE (WSSD) IN PENAEUS INDICUS BY POLYMERASE CHAIN REACTION (PCR) METHOD

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AFSHARNASAB M.; REZVANI S.; LALOUEI F.

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Journal: Iranian Scientific Fisheries Journal Year:2005 | Volume:14 | Issue:1 Page(s): 1-12

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Title

IDENTIFICATION OF WHITE SPOT SYNDROME DISEASE (WSSD) IN PENAEUS INDICUS BY POLYMERASE CHAIN REACTION (PCR) METHOD

Author(s)

AFSHARNASAB M. | REZVANI S. | LALOUEI F. | Issue Writer Certificate

Keywords

SHRIMPQ3

PENAEUS INDICUSQ3

WHITE SPOT SYNDROME DISEASEQ4

PCRQ4

Abstract

A high mortality of cultured SHRIMP PENAEUS INDICUS was spotted in summer 2002 in Khouzestan province, southwestern Iran. White spots with a size of 0.5-2mm was one of the typical external signs of the infected SHRIMPs. Our examination revealed that the cuticle of the SHRIMPs could be easily separated from their epidermis, their hepatopancreas was swollen, their abdomen and intestine were empty and their body colour was reddish. Based on the symptoms, we suspected that WHITE SPOT SYNDROME DISEASE (WSSD) might have caused the mortality. To ascertain our suspicion, we collected 90 infected specimens from the Khouzestan province and another 120 uninfected specimens from Bushehr and Hormozgan provinces in the south and Golestan province in the northeast Iran.After fixing the samples in pure alcohol, we homogenized the samples and extracted their DNA content using phenol-chloroform methods. Using a WSSD kit, we conducted the PCR method which showed the specimens from Khouzestan province (Abadan area) were definitely infected with WSSD while results for samples from other provinces were negative.

Cites

References

CLINICAL SIGNS AND HISTOPATHOLOGY OF WHITE SPOT SYNDROME DISEASE (WSSD) IN CULTURED PENAEUS INDICUS IN KHOUZESTAN PROVINCE

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APA: Copy

AFSHARNASAB, M., REZVANI, S., & LALOUEI, F.. (2005). IDENTIFICATION OF WHITE SPOT SYNDROME DISEASE (WSSD) IN PENAEUS INDICUS BY POLYMERASE CHAIN REACTION (PCR) METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL, 14(1), 1-12. SID. https://sid.ir/paper/4410/en

Vancouver: Copy

AFSHARNASAB M., REZVANI S., LALOUEI F.. IDENTIFICATION OF WHITE SPOT SYNDROME DISEASE (WSSD) IN PENAEUS INDICUS BY POLYMERASE CHAIN REACTION (PCR) METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2005;14(1):1-12. Available from: https://sid.ir/paper/4410/en

IEEE: Copy

M. AFSHARNASAB, S. REZVANI, and F. LALOUEI, “IDENTIFICATION OF WHITE SPOT SYNDROME DISEASE (WSSD) IN PENAEUS INDICUS BY POLYMERASE CHAIN REACTION (PCR) METHOD,” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 14, no. 1, pp. 1–12, 2005, [Online]. Available: https://sid.ir/paper/4410/en

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