SHORT ARTICLE: PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE

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AZARFAR A.; IZADPANAH K.; AFSHARIFAR A.R.; MASUMI M.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2012 | Volume:48 | Issue:3 (191) Page(s): 403-409

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Information Journal Paper

Title

SHORT ARTICLE: PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE

Author(s)

AZARFAR A. | IZADPANAH K. | AFSHARIFAR A.R. | MASUMI M. | Issue Writer Certificate

Keywords

FARSQ3

ZUCCHINI YELLOW MOSAIC VIRUSQ2

POTYVIRUSESQ4

VIRUS PURIFICATIONQ3

PHYLOGENETIC ANALYSISQ2

Abstract

Zucchini yellow mosaic virus (ZYMV (was isolated from squash samples with symptoms of stunting, leaf blistering and distortion of leaf and fruits, collected in cucurbit fields in Kaftarak area (Shiraz) and verified by ELISA test, designated FARS isolate (ZYMV-F). The virus was propagated in squash plants under greenhouse conditions and purified from infected tissues. The antiserum obtained from purified virus was used in serological tests. Complete sequence of the virus genome was determined using mRNA capture kit and PCR with specific primers followed by cloning and sequencing. Following the sequence walking technique a contig of 9591 bp (JN183062) representing the whole genome of the virus was generated. PHYLOGENETIC ANALYSIS based on the nucleotide and amino acid sequence of the whole genome and individual genes showed that ZYMV-F is closest to Israel and Slovakia isolates. Analysis of partial sequence of CP gene showed that ZYMV isolates in Iran are very similar, with FARS and Karaj isolates clustering in the same clade.

Cites

DETECTION OF CUCURBITS INFECTING VIRUSES IN SHIRAZ ZONE AND SUBURBS BY USING SEROLOGICAL AND MOLECULAR METHODS

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APA: Copy

AZARFAR, A., IZADPANAH, K., AFSHARIFAR, A.R., & MASUMI, M.. (2012). SHORT ARTICLE: PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE. IRANIAN JOURNAL OF PLANT PATHOLOGY, 48(3 (191)), 403-409. SID. https://sid.ir/paper/48072/en

Vancouver: Copy

AZARFAR A., IZADPANAH K., AFSHARIFAR A.R., MASUMI M.. SHORT ARTICLE: PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2012;48(3 (191)):403-409. Available from: https://sid.ir/paper/48072/en

IEEE: Copy

A. AZARFAR, K. IZADPANAH, A.R. AFSHARIFAR, and M. MASUMI, “SHORT ARTICLE: PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 48, no. 3 (191), pp. 403–409, 2012, [Online]. Available: https://sid.ir/paper/48072/en

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