TAXONOMIC POSITION OF TWO IRANIAN ISOLATES OF SUGARCANE MOSAIC VIRUS (SCMV) BASED ON SEQUENCE OF THE 3'-REGION OF THE GENOME

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MASOUMI MAHMOUD; ZARE AVA; IZADPANAH K.A.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:1 (169) Page(s): 1-16

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Title

TAXONOMIC POSITION OF TWO IRANIAN ISOLATES OF SUGARCANE MOSAIC VIRUS (SCMV) BASED ON SEQUENCE OF THE 3'-REGION OF THE GENOME

Author(s)

MASOUMI MAHMOUD | ZARE AVA | IZADPANAH K.A. | Issue Writer Certificate

Keywords

SUGARCANE MOSAIC VIRUSQ3

SUGARCANEQ2

CEREAL VIRUSESQ3

POTYVIRUSQ3

IRANQ2

KHUZISTANQ2

Abstract

SUGARCANE MOSAIC VIRUS (SCMV) is a widespread cereal POTYVIRUS in the world. Two SCMV isolates, KhzL66 and KhzQ86, from SUGARCANE plantation in KHUZISTAN were subjected to RT-PCR using potyviral degenerate and specific primers. Amplified segments were cloned and sequenced. The resulting sequences were assembled to yield segments of 1815 nt which included 3´-region of NIb gene, CP gene and 3´-UTR. Comparisons were made between these two isolates and a number of other cereal POTYVIRUSes available in GenBank using MegAlign (DNASTAR) and CLUSTAL X based on alignment of CP-UTR region. Maize dwarf mosaic virus (MDMV), Sorghum mosaic virus (SrMV), Johnson grass mosaic virus (JGMV), IRANian Johnson grass mosaic virus (IJMV) and Zea mosaic virus (ZeMV) were included in multiple alignment. Phylogenetic tree was constructed by Neighbor-joining majority- rule method. In phylogenetic analysis 41 sequences were differentiated into 7 groups (viruses): MDMV, SrMV, JGMV, IJMV, ZeMV and SCMV with two major subgroups I and II. IRANian isolates of SCMV were grouped with isolates of Egypt and South Africa in a subclade in group I. It is speculated that the IRANian isolates have been introduced into this country with SUGARCANE cuttings.

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APA: Copy

MASOUMI, MAHMOUD, ZARE, AVA, & IZADPANAH, K.A.. (2007). TAXONOMIC POSITION OF TWO IRANIAN ISOLATES OF SUGARCANE MOSAIC VIRUS (SCMV) BASED ON SEQUENCE OF THE 3'-REGION OF THE GENOME. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(1 (169)), 1-16. SID. https://sid.ir/paper/48219/en

Vancouver: Copy

MASOUMI MAHMOUD, ZARE AVA, IZADPANAH K.A.. TAXONOMIC POSITION OF TWO IRANIAN ISOLATES OF SUGARCANE MOSAIC VIRUS (SCMV) BASED ON SEQUENCE OF THE 3'-REGION OF THE GENOME. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(1 (169)):1-16. Available from: https://sid.ir/paper/48219/en

IEEE: Copy

MAHMOUD MASOUMI, AVA ZARE, and K.A. IZADPANAH, “TAXONOMIC POSITION OF TWO IRANIAN ISOLATES OF SUGARCANE MOSAIC VIRUS (SCMV) BASED ON SEQUENCE OF THE 3'-REGION OF THE GENOME,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 1 (169), pp. 1–16, 2007, [Online]. Available: https://sid.ir/paper/48219/en

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