NUCLEOTIDE SEQUENCE OF HOP STUNT VIROID CDNA FROM CACHEXIAINFECTED CITRUS PLANTS IN MAZANDARAN PROVINCE

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ALAVI S.M.; AHOUNMANESH A.; RAHIMIAN H.A.

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Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:1 (169) Page(s): 49-64

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Title

NUCLEOTIDE SEQUENCE OF HOP STUNT VIROID CDNA FROM CACHEXIAINFECTED CITRUS PLANTS IN MAZANDARAN PROVINCE

Author(s)

ALAVI S.M. | AHOUNMANESH A. | RAHIMIAN H.A. | Issue Writer Certificate

Keywords

CACHEXIAQ3

STEM PITTINGQ3

HOP STUNTQ3

VIROIDQ2

UNSHIUQ3

Abstract

CACHEXIA is a VIROID disease of citrus worldwide. Field symptoms of CACHEXIA including stunting, chlorosis, decline, gumming of the bark and STEM PITTING have been observed on many Satsuma (Citrus UNSHIU) and Clementine (C. reticulata) and some sweet orange trees in Mazandaran. In 2004, eight Satsuma and Clementine samples were collected from symptomatic trees in eastern Mazandaran. Young leaves and bark tissues were used for RNA extraction. Reverse transcription-polymerase chain reaction was performed using VIROID specific primer pairs of HOP STUNT VIROID, Citrus bent leaf VIROID, Citrus exocortis VIROID, Citrus VIROID III, Citrus VIROID IV and coat protein gene (CP25) of Citrus tristeza virus. The expected HSVd 300bp fragment was observed on 1.5% agarose gels following electrophoresis. Single-strand conformation polymorphism analysis differentiated the isolates into two groups. The amplified products from a representative of both groups were ligated into pBluescript SK and one clone of each was sequenced using M13 primers. Alignment of the nucleotide sequences of the isolates with those deposited in GenBank revealed their highest identities with other isolates of HOP STUNT VIROID. As the symptoms of citrus CACHEXIA disease have been observed in almost all citrus trees sampled, it appears that the majority of citrus trees in northern Iran are infected with the HSVd. This is the first report on the molecular characterization of HOP STUNT VIROID in citrus CACHEXIA disease from Iran.

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APA: Copy

ALAVI, S.M., AHOUNMANESH, A., & RAHIMIAN, H.A.. (2007). NUCLEOTIDE SEQUENCE OF HOP STUNT VIROID CDNA FROM CACHEXIAINFECTED CITRUS PLANTS IN MAZANDARAN PROVINCE. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(1 (169)), 49-64. SID. https://sid.ir/paper/48220/en

Vancouver: Copy

ALAVI S.M., AHOUNMANESH A., RAHIMIAN H.A.. NUCLEOTIDE SEQUENCE OF HOP STUNT VIROID CDNA FROM CACHEXIAINFECTED CITRUS PLANTS IN MAZANDARAN PROVINCE. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(1 (169)):49-64. Available from: https://sid.ir/paper/48220/en

IEEE: Copy

S.M. ALAVI, A. AHOUNMANESH, and H.A. RAHIMIAN, “NUCLEOTIDE SEQUENCE OF HOP STUNT VIROID CDNA FROM CACHEXIAINFECTED CITRUS PLANTS IN MAZANDARAN PROVINCE,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 1 (169), pp. 49–64, 2007, [Online]. Available: https://sid.ir/paper/48220/en

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