STUDIES ON POPULATIONS OF RHIZOCTONLA SOLANI AG-I-IA ISOLATED FROM RICE BY RDNA RFLP IN MAZANDARAN PROVINCE, IRAN

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TAJIK GHANBARI M.A.; RAHIMIAN H.A.; ALIZADEH AZIZ ELAH

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2005 | Volume:41 | Issue:4 Page(s): 507-522

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Information Journal Paper

Title

STUDIES ON POPULATIONS OF RHIZOCTONLA SOLANI AG-I-IA ISOLATED FROM RICE BY RDNA RFLP IN MAZANDARAN PROVINCE, IRAN

Author(s)

TAJIK GHANBARI M.A. | RAHIMIAN H.A. | ALIZADEH AZIZ ELAH | Issue Writer Certificate

Keywords

RICEQ4

SHEATH BLIGHTQ4

RHIZOCTONIA SOLANI AG-I-IAQ2

RDNA RFLPQ2

PCRQ2

Abstract

Fifty four isolates of RHIZOCTONIA SOLANI AG-I-IA the causal agent of RICE SHEATH BLIGHT disease from RICE fields of Mazandaran province were isolated and analyzed for probable polymorphism ofITS-5.8S rDNA region by PCR based RFLP.Genomic DNA was extracted from isolates and amplified by PCR, using internal transcribed spacer (ITS) primers. Primer pairs ITS4 and ITS5 were used and the PCR products were resolved on 8% polyacrylamide gel and stained with ethidium bromide.The isolates were differentiated into two groups on the basis of the size and number of fragments amplified. One group of isolates produced a 700 bp fragment and members of the other group displayed three bands, 700, 850 and 950 bp in size.To assess existence of any further polymorphism in ITS region, the PCR products were digested with restriction endonucleases BamHI, Hind Ill, XhoII, HaeIII, XhoI, EcoRI and Taq1.BamHI, HindIII and XhoI did not have recognition sequences on the amplified products EcoRI, HaeIII, TaqI and XhoII cut the amplified ITS regions into two or three fragments but the digested products were similar among the isolates in each of the two groups. Thus no further polymorphism was discernible.

Cites

PATHOGENIC VARIABILITY AND GENETIC DIVERSITY OF FUSARIUM SOLANI ISOLATES AND ITS ASSOCIATION WITH SUGARBEET ROOT ROT

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APA: Copy

TAJIK GHANBARI, M.A., RAHIMIAN, H.A., & ALIZADEH, AZIZ ELAH. (2005). STUDIES ON POPULATIONS OF RHIZOCTONLA SOLANI AG-I-IA ISOLATED FROM RICE BY RDNA RFLP IN MAZANDARAN PROVINCE, IRAN. IRANIAN JOURNAL OF PLANT PATHOLOGY, 41(4), 507-522. SID. https://sid.ir/paper/48258/en

Vancouver: Copy

TAJIK GHANBARI M.A., RAHIMIAN H.A., ALIZADEH AZIZ ELAH. STUDIES ON POPULATIONS OF RHIZOCTONLA SOLANI AG-I-IA ISOLATED FROM RICE BY RDNA RFLP IN MAZANDARAN PROVINCE, IRAN. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2005;41(4):507-522. Available from: https://sid.ir/paper/48258/en

IEEE: Copy

M.A. TAJIK GHANBARI, H.A. RAHIMIAN, and AZIZ ELAH ALIZADEH, “STUDIES ON POPULATIONS OF RHIZOCTONLA SOLANI AG-I-IA ISOLATED FROM RICE BY RDNA RFLP IN MAZANDARAN PROVINCE, IRAN,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 41, no. 4, pp. 507–522, 2005, [Online]. Available: https://sid.ir/paper/48258/en

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