GENETIC DIVERSITY OF SUGAR BEET ISOLATES OF RHIZOCTONIA SOLANI REVEALED BY RAPD-PCR AND ITS-RDNA ANALYSIS

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MAHMOUDI B.; MESBAH M.; RAHIMIAN H.; ALI ZADEH A.; NORUZI P.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2005 | Volume:41 | Issue:4 Page(s): 523-542

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Title

GENETIC DIVERSITY OF SUGAR BEET ISOLATES OF RHIZOCTONIA SOLANI REVEALED BY RAPD-PCR AND ITS-RDNA ANALYSIS

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Keywords

GENETIC DIVERSITYQ2

ITS-RDNAQ4

RAPD-PCRQ2

RHIZOCTONIA SOLANIQ4

SUGAR BEETQ4

Abstract

GENETIC DIVERSITY of Iranian isolates of R. solani associated with SUGAR BEET root rot was studied using RAPD-PCR and ITS-RDNA analysis. Twelve of the 14 random oligo-primers used in RAPD-PCR yielded scorable polymorphism among the isolates. The DNA bands on agarose and polyacrylamide gels were scored using binary code and a similarity matrix based on simple matching coefficient, was generated using SPSS software. No correlation was found between the RAPD profiles and the geographic location and virulence level of, or the type of disease caused (host, tissue affected) by the isolates of R. salani. The isolates belonging to different anastomosis groups could be distinguished at 85% similarity level. A DNA fragment of 650-750 bp in size was amplified from DNA preparations of all isolates with the ITS4 and ITS5 primers. The fragments possessed no recognition site for endonuclease Pst1. The RFLP profiles generated through digestion of the amplified products with EcaRl and Tru91 were useful in differentiating the isolates of different anastomosis groups.

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APA: Copy

MAHMOUDI, B., MESBAH, M., RAHIMIAN, H., ALI ZADEH, A., & NORUZI, P.. (2005). GENETIC DIVERSITY OF SUGAR BEET ISOLATES OF RHIZOCTONIA SOLANI REVEALED BY RAPD-PCR AND ITS-RDNA ANALYSIS. IRANIAN JOURNAL OF PLANT PATHOLOGY, 41(4), 523-542. SID. https://sid.ir/paper/48259/en

Vancouver: Copy

MAHMOUDI B., MESBAH M., RAHIMIAN H., ALI ZADEH A., NORUZI P.. GENETIC DIVERSITY OF SUGAR BEET ISOLATES OF RHIZOCTONIA SOLANI REVEALED BY RAPD-PCR AND ITS-RDNA ANALYSIS. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2005;41(4):523-542. Available from: https://sid.ir/paper/48259/en

IEEE: Copy

B. MAHMOUDI, M. MESBAH, H. RAHIMIAN, A. ALI ZADEH, and P. NORUZI, “GENETIC DIVERSITY OF SUGAR BEET ISOLATES OF RHIZOCTONIA SOLANI REVEALED BY RAPD-PCR AND ITS-RDNA ANALYSIS,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 41, no. 4, pp. 523–542, 2005, [Online]. Available: https://sid.ir/paper/48259/en

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