COMBINED ANALYSIS OF GENETIC AND RESISTANCE GENE DIVERSITIES IN WHEAT CULTIVARS USING RGA MOLECULAR MARKER

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HABIBZADEH ALI REZA; KESHAVARZI MANSOUREH; AFSHARI FARZAD; NAGHAVI M.R.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:3 (171) Page(s): 327-337

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Title

COMBINED ANALYSIS OF GENETIC AND RESISTANCE GENE DIVERSITIES IN WHEAT CULTIVARS USING RGA MOLECULAR MARKER

Author(s)

HABIBZADEH ALI REZA | KESHAVARZI MANSOUREH | AFSHARI FARZAD | NAGHAVI M.R. | Issue Writer Certificate

Keywords

RGAQ3

WHEATQ2

GENE DIVERSITIESQ2

Abstract

Comparisons of nucleotide sequences of genes cloned from diverse plants for resistance to different pathogens indicate presence of conserved domains called resistance gene analogs (RGA) in these genes. Based on these conserved regions, degenerate primers have been designed and used to study defense mechanisms and to clone resistance genes. In this study, five pairs of degenerate RGA primers were used to detect genetic and resistance GENE DIVERSITIES in 30 local and foreign WHEAT cultivars. Results indicated sharp banding of PCR products in denaturing polyacrylamide gels electroforesed for 2-2.5 hrs at 1600 Volts in 400x400x0.4 mm apparatus. Totally 1486 bands with 33-42% polymorphism were detected.The local cultivars were clustered in a separate cluster. In the cluster of local cultivars, the position of each cultivar was related to its pedigree. Within the dendrogram, the most susceptible cultivars to yellow rust clustered together. The results indicate efficiency of RGA approach to study combined diversity of genetic candidate resistance genes. The analysis of the data is however somewhat complicated, due to the participation of two different properties in the clustering.

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APA: Copy

HABIBZADEH, ALI REZA, KESHAVARZI, MANSOUREH, AFSHARI, FARZAD, & NAGHAVI, M.R.. (2007). COMBINED ANALYSIS OF GENETIC AND RESISTANCE GENE DIVERSITIES IN WHEAT CULTIVARS USING RGA MOLECULAR MARKER. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(3 (171)), 327-337. SID. https://sid.ir/paper/48352/en

Vancouver: Copy

HABIBZADEH ALI REZA, KESHAVARZI MANSOUREH, AFSHARI FARZAD, NAGHAVI M.R.. COMBINED ANALYSIS OF GENETIC AND RESISTANCE GENE DIVERSITIES IN WHEAT CULTIVARS USING RGA MOLECULAR MARKER. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(3 (171)):327-337. Available from: https://sid.ir/paper/48352/en

IEEE: Copy

ALI REZA HABIBZADEH, MANSOUREH KESHAVARZI, FARZAD AFSHARI, and M.R. NAGHAVI, “COMBINED ANALYSIS OF GENETIC AND RESISTANCE GENE DIVERSITIES IN WHEAT CULTIVARS USING RGA MOLECULAR MARKER,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 3 (171), pp. 327–337, 2007, [Online]. Available: https://sid.ir/paper/48352/en

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