ISOLATION OF CROWN GALL AGENT AND EFFECT OF THERMO-CHEMOTHERAPY ON ITS ELIMINATION FROM GRAPEVINE CUTTINGS

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MAHMOUDZADEH HASAN; DAVOUDI ABAS

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:4 (172) Page(s): 410-428

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Title

ISOLATION OF CROWN GALL AGENT AND EFFECT OF THERMO-CHEMOTHERAPY ON ITS ELIMINATION FROM GRAPEVINE CUTTINGS

Author(s)

MAHMOUDZADEH HASAN | DAVOUDI ABAS | Issue Writer Certificate

Keywords

THERMOTHERAPYQ4

CHEMOTHERAPYQ3

CROWN GALLQ3

GRAPE SENSITIVE CULTIVARSQ3

Abstract

In order to isolation of grape CROWN GALL agent and study on effect of hot water and chemical treatments on reducing of bacterium from infected cuttings and some characteristics of rooted cuttings cvs. Sefid Bedaneh and Hossaini, an experiment was conducted in Grape Research Station of Takestan (3 years). Experiment was done as Split-Split plot (based on CRBD, 4 replications, 40 samples in plot). Isolation and identification of bacteria isolates was done by using common methods such as selective media and test on indicator plants. Treatments were cultivars (Main Factor), Hot-Water (sub-plot) and chemical treatments (subsub plot). Cuttings were rooted in disinfected mixed of Perlite, sand and compost soil (isolated nursery). 3 years duration, effects of treatments on deleting of infection were studied by observation of gall formation and bacterium growing on selective media. Analysis of variance was done by MSTAT-C program and mean comparison done by LSD method. 17 strains of 20 isolates were had Agrobacterium tumefaciens characteristics and 3 of them were A. vitis. Effect of combined treatments on vitality and growth characteristics varied with different cultivars and treatments. The number of healthy rooted cuttings, cuttings survival, roots fresh weight and weight of shoots were varied. Also, flower initiation was affected by treatments in two cultivars. Results showed that agent of CROWN GALL was eradicated or reduced below the level of detection in dormant cuttings of two cvs of grapes by combined Hot water treatment (50°C/30 min)× chemical treatment (Streptomycin Sulfate 1000 ppm). Although, 60°C/15 min treatment was more effective treatment, but it killed high number of cuttings. Rooting of cuttings, fruit production and other characteristics were better by combined (50°C/30 min) × chemical treatment (Streptomycin Sulfate 1000 ppm).

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APA: Copy

MAHMOUDZADEH, HASAN, & DAVOUDI, ABAS. (2007). ISOLATION OF CROWN GALL AGENT AND EFFECT OF THERMO-CHEMOTHERAPY ON ITS ELIMINATION FROM GRAPEVINE CUTTINGS. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(4 (172)), 410-428. SID. https://sid.ir/paper/48376/en

Vancouver: Copy

MAHMOUDZADEH HASAN, DAVOUDI ABAS. ISOLATION OF CROWN GALL AGENT AND EFFECT OF THERMO-CHEMOTHERAPY ON ITS ELIMINATION FROM GRAPEVINE CUTTINGS. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(4 (172)):410-428. Available from: https://sid.ir/paper/48376/en

IEEE: Copy

HASAN MAHMOUDZADEH, and ABAS DAVOUDI, “ISOLATION OF CROWN GALL AGENT AND EFFECT OF THERMO-CHEMOTHERAPY ON ITS ELIMINATION FROM GRAPEVINE CUTTINGS,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 4 (172), pp. 410–428, 2007, [Online]. Available: https://sid.ir/paper/48376/en

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