INFECTIVITY OF THE CLONED GENOME OF IRANIAN ISOLATE OF BEET SEVERE CURLY TOP VIRUS IN EXPERIMENTAL HOSTS

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EBADZAD SAHRAEI GHAZAL; BAHJATNIA S.A.A.; IZADPANAH K.A.

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ISSN: 2588-4824

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2008 | Volume:44 | Issue:2 (174) Page(s): 176-183

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Information Journal Paper

Title

INFECTIVITY OF THE CLONED GENOME OF IRANIAN ISOLATE OF BEET SEVERE CURLY TOP VIRUS IN EXPERIMENTAL HOSTS

Author(s)

EBADZAD SAHRAEI GHAZAL | BAHJATNIA S.A.A. | IZADPANAH K.A. | Issue Writer Certificate

Keywords

GEMINVIRUSQ2

BEET CURLY TOP VIRUSQ3

BEET SEVERE CURLY TOP VIRUSQ2

INFECTIOUS CLONEQ3

CURTOVIRUSQ3

Abstract

To construct the INFECTIOUS CLONE and to test the infectivity of the full-length DNA of Iranian isolate of BEET SEVERE CURLY TOP VIRUS (BSCTV-Ir), a 1942 bp SphI/EcoRI fragment of the virus (0.7 genome) was cloned into vector pTZ57R. The resulting construct was digested with SphI and the complete monomer DNA of the virus was cloned into it to produce the partial dimer construct containing the tandem-repeat (1.7) of BSCTV-Ir genome. This DNA was ligated into the pBin20 and transferred into Agrobacterium tumefaciens strain C58 and inoculated into several plants. Typical curly top symptoms were observed on newly developed leaves of beet plants within 30 to 35 days postinoculation. Agroinoculated tomato, spinach and Jimson weed plants showed yellowing and leaf curling. PCR analysis using BSCTV-Ir-specific primers, designed to amplify the viral coat protein, showed the presence of BSCTV-Ir DNA in all inoculated plants and proved the infectivity of constructed BSCTV-Ir INFECTIOUS CLONE. This construct can be used as a source of BSCTV-Ir in various studies including screening of resistant cultivars.

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APA: Copy

EBADZAD SAHRAEI, GHAZAL, BAHJATNIA, S.A.A., & IZADPANAH, K.A.. (2008). INFECTIVITY OF THE CLONED GENOME OF IRANIAN ISOLATE OF BEET SEVERE CURLY TOP VIRUS IN EXPERIMENTAL HOSTS. IRANIAN JOURNAL OF PLANT PATHOLOGY, 44(2 (174)), 176-183. SID. https://sid.ir/paper/48390/en

Vancouver: Copy

EBADZAD SAHRAEI GHAZAL, BAHJATNIA S.A.A., IZADPANAH K.A.. INFECTIVITY OF THE CLONED GENOME OF IRANIAN ISOLATE OF BEET SEVERE CURLY TOP VIRUS IN EXPERIMENTAL HOSTS. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2008;44(2 (174)):176-183. Available from: https://sid.ir/paper/48390/en

IEEE: Copy

GHAZAL EBADZAD SAHRAEI, S.A.A. BAHJATNIA, and K.A. IZADPANAH, “INFECTIVITY OF THE CLONED GENOME OF IRANIAN ISOLATE OF BEET SEVERE CURLY TOP VIRUS IN EXPERIMENTAL HOSTS,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 44, no. 2 (174), pp. 176–183, 2008, [Online]. Available: https://sid.ir/paper/48390/en

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