GENE 18S RRNA VARIATION OF CUTTLEFISH POPULATION (SEPIA PHARAONIS) IN THE PERSIAN GULF AND THE OMAN SEA USING PCR-RFLP METHOD

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NAHAVANDI R.; REZVANI S.; VOSOUGHI GHOLAM HOSSEIN; KAZEMI B.

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Journal: Iranian Scientific Fisheries Journal Year:2005 | Volume:14 | Issue:2 Page(s): 157-168

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Information Journal Paper

Title

GENE 18S RRNA VARIATION OF CUTTLEFISH POPULATION (SEPIA PHARAONIS) IN THE PERSIAN GULF AND THE OMAN SEA USING PCR-RFLP METHOD

Author(s)

NAHAVANDI R. | REZVANI S. | VOSOUGHI GHOLAM HOSSEIN | KAZEMI B. | Issue Writer Certificate

Keywords

PCR-RFLPQ3

CUTTLEFISHQ3

RRNAQ3

PERSIAN GULFQ4

OMAN SEAQ3

Abstract

We used PCR-RFLP method to identify CUTTLEFISH (Sepia pharaonis) populations in the PERSIAN GULF and the Sea of Oman. Bottom trawling method was used to collect a range of 20 to 40 specimens from each 15 stations in the study area. Genomic DNA was extracted by phenol-chloroform method and one pair primer was designed for the analysis based on 18s RRNA gene nucleotide sequences. A PCR product with 502 pair bases in length was obtained for all specimens and subjected to digestion by eight restriction enzymes AluI,TaqI, MnlI, RsaI, HindIII, DraI, PvuII and HaeIII. DNA banding patterns in all specimens were similar and no polymorphism was detected among them. We conclude that CUTTLEFISH populations cannot be isolated using 18s RRNA gene extracts in the area of study.

Cites

THE STUDY OF EMBRYONIC DEVELOPMENT AND HATCHING SQUID PRAWNS (SEPIA PHARAONIS) IN THE OMAN SEA (CHABAHAR)

References

MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD

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APA: Copy

NAHAVANDI, R., REZVANI, S., VOSOUGHI, GHOLAM HOSSEIN, & KAZEMI, B.. (2005). GENE 18S RRNA VARIATION OF CUTTLEFISH POPULATION (SEPIA PHARAONIS) IN THE PERSIAN GULF AND THE OMAN SEA USING PCR-RFLP METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL, 14(2), 157-168. SID. https://sid.ir/paper/5013/en

Vancouver: Copy

NAHAVANDI R., REZVANI S., VOSOUGHI GHOLAM HOSSEIN, KAZEMI B.. GENE 18S RRNA VARIATION OF CUTTLEFISH POPULATION (SEPIA PHARAONIS) IN THE PERSIAN GULF AND THE OMAN SEA USING PCR-RFLP METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2005;14(2):157-168. Available from: https://sid.ir/paper/5013/en

IEEE: Copy

R. NAHAVANDI, S. REZVANI, GHOLAM HOSSEIN VOSOUGHI, and B. KAZEMI, “GENE 18S RRNA VARIATION OF CUTTLEFISH POPULATION (SEPIA PHARAONIS) IN THE PERSIAN GULF AND THE OMAN SEA USING PCR-RFLP METHOD,” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 14, no. 2, pp. 157–168, 2005, [Online]. Available: https://sid.ir/paper/5013/en

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