DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION

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KARGAR MOAKHAR R.; GHOREYSHI S.A.; SADEGHI M.R.; MORSHEDI D.; MASOUDI S.; POURBAKHSH S.A.

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Journal Paper

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2003 | Volume:- | Issue:55 Page(s): 11-18

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Title

DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION

Author(s)

Keywords

BOVINE HERPESVIRUS 1

GI GENE

POLYMERASE CHAIN REACTION

IRAN

Abstract

Based on primers from gI glycoprotein, a POLYMERASE CHAIN REACTION (PCR) assay was optimized for detection of bovine herpes virus type 1 (BHV-1). A 468bp DNA fragment of nine isolates was amplified. The PCR products were detected by ethidium bromide staining after gel electrophoresis and confirmed by sequencing. The nucleotide sequence alignments revealed a highly conserved region in GI GENE within the isolates. Results suggest that PCR can be used as a reliable diagnostic tool for rapid detection of BHV-1 infections.

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APA: Copy

KARGAR MOAKHAR, R., GHOREYSHI, S.A., SADEGHI, M.R., MORSHEDI, D., MASOUDI, S., & POURBAKHSH, S.A.. (2003). DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION. ARCHIVES OF RAZI INSTITUTE, -(55), 11-18. SID. https://sid.ir/paper/528047/en

Vancouver: Copy

KARGAR MOAKHAR R., GHOREYSHI S.A., SADEGHI M.R., MORSHEDI D., MASOUDI S., POURBAKHSH S.A.. DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION. ARCHIVES OF RAZI INSTITUTE[Internet]. 2003;-(55):11-18. Available from: https://sid.ir/paper/528047/en

IEEE: Copy

R. KARGAR MOAKHAR, S.A. GHOREYSHI, M.R. SADEGHI, D. MORSHEDI, S. MASOUDI, and S.A. POURBAKHSH, “DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 55, pp. 11–18, 2003, [Online]. Available: https://sid.ir/paper/528047/en

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